Population Diversity of Antibiotic Resistant in Samples From Wildlife Origin in Senegal: Identification of a Multidrug Resistance Transposon Carrying in .

INTRODUCTION

The role of wildlife in the transmission of antimicrobial resistant (AMR) is suspected but scarcely reported in current studies. Therefore, we studied the dynamics and prevalence of antibiotic-resistant in antibiotic-limited areas of Senegal.

MATERIALS AND METHODS

We collected fecal samples from monkeys and apes (N = 226) and non-fecal environmental samples (N = 113) from Senegal in 2015 and 2019. We grew the samples on selective media, subsequently isolated AMR , and then sequenced their genomes.

RESULTS

We isolated 72 different among which we obtained a resistance rate of 65% for colistin (N = 47/72) and 29% for third generation-cephalosporin (C3G) (29%, N = 21/72). Interestingly, almost 46% of our isolates, among sp., and , belong to 34 new STs. Moreover, the genes , , , , , (), (), , and harbored within a transposon on the plasmid of ST224 were transferred and inserted into a ST10 . phage coding region.

CONCLUSION

Wildlife constitutes a rich, unexplored reservoir of natural microbial diversity, AMR genes and international resistant clones pathogenic in humans. The presence of a transposon that carries AMR genes is intriguing since no antibiotics are used in the non-human primates we studied.