Jones R B, Katz B P, van der Pol B, Caine V A, Batteiger B E, Newhall W J
J Clin Microbiol. 1986 Dec;24(6):1029-33. doi: 10.1128/jcm.24.6.1029-1033.1986.
Detection of chlamydial infections depends on the sensitivity of the techniques used. Variables include the number of body sites sampled, the number of samples obtained, and the number of passages in tissue culture. To assess these factors, microdilution plate cultures with a single blind passage were performed on specimens from 10,291 men and women attending a sexually transmitted disease clinic. Overall, 21% of the men and 30% of the women were culture positive. However, 18% of endocervical, 28% of female urethral, and 29% of male urethral cultures that were positive became so only after a single passage. Of culture-positive women, 23% were positive at the urethra only. Pooled urethral and endocervical specimens were positive more often than an endocervical specimen alone but less often than separately cultured endocervical and urethral specimens. A total of 221 specimens from 92 men and 66 women were subjected to five serial blind passages. Of 83 positive specimens, 29 (35%) were positive only after two or more passages. A total of 37 (46%) women were culture-positive, but only 12 (33%) of those who were positive and had an endocervical culture would have been detected by a single endocervical culture that was not passaged. The sensitivity of chlamydial culture is substantially less than 100% but can be improved by culturing samples from both the urethra and endocervix in women and by serial passage in tissue culture.
衣原体感染的检测取决于所使用技术的敏感性。变量包括采样的身体部位数量、获取的样本数量以及组织培养中的传代次数。为评估这些因素，对一家性传播疾病诊所的10291名男性和女性的标本进行了单盲传代的微量稀释平板培养。总体而言，21%的男性和30%的女性培养呈阳性。然而，18%的宫颈内膜、28%的女性尿道和29%的男性尿道培养物仅在单次传代后才呈阳性。在培养呈阳性的女性中，23%仅尿道呈阳性。合并的尿道和宫颈内膜标本比单独的宫颈内膜标本更常呈阳性，但比分别培养的宫颈内膜和尿道标本更不常呈阳性。对来自92名男性和66名女性的221份标本进行了五次连续盲传代。在83份阳性标本中，29份（35%）仅在两次或更多次传代后才呈阳性。共有37名（46%）女性培养呈阳性，但在呈阳性且进行了宫颈内膜培养的女性中，只有12名（33%）能通过未传代的单次宫颈内膜培养检测出来。衣原体培养的敏感性远低于100%，但通过对女性尿道和宫颈内膜的样本进行培养以及在组织培养中进行连续传代可以提高敏感性。
