Yu Caiyun, Wang Dong, Tong Yichun, Li Qiming, Yang Weiren, Wang Tian, Yang Zaibin
College of Animal Sciences and Technology, Nanjing Agricultural University, Nanjing, China.
Heilongjiang Key Laboratory of Experimental Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Front Microbiol. 2022 Mar 21;13:831882. doi: 10.3389/fmicb.2022.831882. eCollection 2022.
This study investigated the alleviative potential of -anethole (TA) on the impaired intestinal barrier and intestinal inflammation and its regulatory effects on gut microbiota in broilers with subclinical necro-hemorrhagic enteritis (NE) challenge. Subclinical NE challenge led to a severe decline in the 21-day body weight (BW) and average daily gain (ADG), but an increase in feed conversion ratio (FCR) and intestinal lesion score of birds compared with controls ( < 0.05). Compared with the subclinical NE group, the TA administration group exhibited lower ( < 0.05) intestinal lesion score and crypt depth (CD), serum diamine oxidase activity, and D-lactate concentration, but higher ( < 0.05) intestinal tight junction protein expressions, villus height (VH), VH/CD, and numbers of proliferating cell nuclear antigen (PCNA)-positive cells. The administration of TA also inhibited ( < 0.05) the expression of intestinal pro-inflammatory cytokines including interleukin (IL)-1β, IL-8, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) but increased ( < 0.05) jejunal IL-10 and secretory immunoglobulin A (sIgA) concentration. TA inclusion also led to a remarkable reduction of intestinal NF-kappa-B inhibitor alpha (IκBα) degradation and nuclear factor kappa beta (NF-κB) translocation. Moreover, TA modulated the cecal microbiota abundance and diversity of NE birds, as confirmed by reducing the phylum Firmicutes and genera , _, and when supplemented at 600 mg/kg and reducing genera , , and when supplemented at 400 mg/kg ( < 0.05). Supplementation of TA in broiler diets could alleviate subclinical NE infection by restoring intestinal barrier integrity, inhibiting NF-κB signaling pathway, and modulating gut microbiota. A 600-mg/kg dose of TA is the optimum concentration for ameliorating subclinical NE in broilers.
本研究探讨了反式茴香脑（TA）对亚临床坏死性出血性肠炎（NE）攻毒肉鸡肠道屏障受损和肠道炎症的缓解潜力及其对肠道微生物群的调节作用。亚临床NE攻毒导致21日龄体重（BW）和平均日增重（ADG）严重下降，但与对照组相比，鸡的饲料转化率（FCR）和肠道病变评分增加（P<0.05）。与亚临床NE组相比，TA给药组的肠道病变评分和隐窝深度（CD）、血清二胺氧化酶活性和D-乳酸浓度较低（P<0.05），但肠道紧密连接蛋白表达、绒毛高度（VH）、VH/CD和增殖细胞核抗原（PCNA）阳性细胞数量较高（P<0.05）。TA的给药还抑制（P<0.05）了包括白细胞介素（IL）-1β、IL-8、干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）在内的肠道促炎细胞因子的表达，但增加了（P<0.05）空肠IL-10和分泌型免疫球蛋白A（sIgA）浓度。添加TA还导致肠道NF-κB抑制因子α（IκBα）降解和核因子κB（NF-κB）易位显著减少。此外，TA调节了NE鸡盲肠微生物群的丰度和多样性，600 mg/kg添加量时，厚壁菌门以及属、、的丰度降低，当400 mg/kg添加时，属、、丰度降低（P<0.05）得到证实。在肉鸡日粮中添加TA可通过恢复肠道屏障完整性、抑制NF-κB信号通路和调节肠道微生物群来缓解亚临床NE感染。600 mg/kg剂量的TA是改善肉鸡亚临床NE的最佳浓度。
