Wang Danni, Wang He
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai Shanghai China.
Department of Microbiology Guizhou Medical University Guiyang Guizhou China.
Health Sci Rep. 2022 Apr 1;5(3):e574. doi: 10.1002/hsr2.574. eCollection 2022 Apr.
The prostatic secretion was considered to be the most important and even only specimen in diagnosis and differential diagnosis of chronic prostatitis like symptoms, but little attention has been paid to other genital organ infections. A urine-prostate-semen test (U-EPS-S test) was used to investigate the microbial flora of internal genital organs in patients with chronic prostatitis-like symptoms and their influence on the diagnosis and treatment of the patients.
We randomly selected the patients with chronic prostatitis-like symptoms for this study and used a U-EPS-S test to collect urine, prostatic secretion, and semen specimens. The specimens were inoculated respectively into a suitable culture medium for bacteriological/fungal detection, and the number and distribution of colonies in each isolation culture were observed before and after the therapy.
All of the specimens from the internal genital organs of these patients were shown as microbe-positive isolation and the infection rate was 100%. Of these, prostatic secretion with microbe-positive isolation was obtained in 66 cases (33%), semen with microbe-positive isolation was obtained in 34 cases (17%), and both prostatic secretion and semen with microbe-positive isolation were obtained in 100 cases (50%). In the isolates, Gram-positive microbes were shown as the most common pathogens, accounting for 91.1%. In 200 patients, 95 patients were infected with one microbial species infection, of them 36 were prostatic secretion positive-isolation (18%), 20 were semen sample positive-isolation (10%), and 39 were positive-isolation both prostatic secretion and semen samples (19.5%); 104 patients were infected with two microbial species, of them 30 were prostatic secretion positive-isolation (15%), 14 were semen sample positive-isolation (7%), and 60 were positive-isolation both prostatic secretion and semen samples (30%); one patient was infected with three microbial species and them were isolated from the semen sample (0.5%). In the patients with chronic prostatitis-like symptoms, the multiple microbial infection (MMI) was accounted for 53.5%, and the multiorgan infection (MOI) was accounted for 67%.
The U-EPS-S test is not only helpful to accurately identify the pathogens and contaminants in the culture isolates, but also the diagnosis and differential diagnosis and also evaluation of the treatment efficacy of the infection in different genital organs. In the patients with chronic prostatitis symptoms, Gram-positive microbes were the most common causative agents, and MMI and MOI caused by resistant strains of different microbial species have a high incidence.
前列腺分泌物曾被认为是慢性前列腺炎样症状诊断及鉴别诊断中最重要甚至是唯一的标本，但其他生殖器官感染却很少受到关注。采用尿液-前列腺液-精液检测（U-EPS-S检测）来研究慢性前列腺炎样症状患者内生殖器官的微生物菌群及其对患者诊断和治疗的影响。
我们随机选取慢性前列腺炎样症状患者进行本研究，采用U-EPS-S检测收集尿液、前列腺液和精液标本。将标本分别接种于合适的培养基中进行细菌学/真菌检测，并观察治疗前后各分离培养物中菌落的数量及分布情况。
这些患者内生殖器官的所有标本均显示为微生物阳性分离，感染率为100%。其中，前列腺液微生物阳性分离的有66例（33%），精液微生物阳性分离的有34例（17%），前列腺液和精液微生物均阳性分离的有100例（50%）。在分离株中，革兰氏阳性微生物是最常见的病原体，占91.1%。200例患者中，95例为单一微生物感染，其中前列腺液阳性分离的有36例（18%），精液标本阳性分离的有20例（10%），前列腺液和精液标本均阳性分离的有39例（19.5%）；104例患者为两种微生物感染，其中前列腺液阳性分离的有30例（15%），精液标本阳性分离的有14例（7%），前列腺液和精液标本均阳性分离的有60例（30%）；1例患者为三种微生物感染，且均从精液标本中分离得到（0.5%）。慢性前列腺炎样症状患者中，多重微生物感染（MMI）占53.5%，多器官感染（MOI）占67%。
U-EPS-S检测不仅有助于准确鉴定培养分离物中的病原体和污染物，还对不同生殖器官感染的诊断、鉴别诊断及治疗效果评估有帮助。在慢性前列腺炎症状患者中，革兰氏阳性微生物是最常见的病原体，不同微生物耐药菌株引起的MMI和MOI发生率较高。
