Dunkić Valerija, Nazlić Marija, Ruščić Mirko, Vuko Elma, Akrap Karla, Topić Snježana, Milović Milenko, Vuletić Nenad, Puizina Jasna, Jurišić Grubešić Renata, Srečec Siniša, Kremer Dario
Faculty of Science, University of Split, Rudera Boškovića 33, 21000 Split, Croatia.
Antun Vrančić Grammar School, Put Gimnazije 64, 22000 Šibenik, Croatia.
Plants (Basel). 2022 Mar 28;11(7):902. doi: 10.3390/plants11070902.
Free volatile compounds were isolated from 21 Croatian species studied by hydrodistillation (HD) and microwave extraction (ME) and analyzed by gas chromatography coupled with mass spectrometry. Principal Component Analysis (PCA) distinguished some clusters based on the relative proportion of major compounds, such as hexadecanoic acid, hexahydrofarnesyl acetone, phytol, -caryophyllene, and caryophyllene oxide, which were identified in all species studied by both isolation methods. In addition to these compounds, germacrene D, δ-selinene, and eicosane were also identified in five samples from dry habitats isolated using ME. -aromadendrene and β-ionone are particularly abundant in five species from wet habitats isolated by both methods. The peculiarities of species from moderate habitats isolated with HD are benzene acetaldehyde, -nonanal, and the identification of significant compounds from the hydrocarbon class, while the peculiarity of ME is ()-β-damascenone. In this article, we present new results on the phytochemical characterization of species from different habitats. The biological potential of these compounds should be further investigated for a better understanding and utilization of the specialized plant metabolites.
通过水蒸馏（HD）和微波萃取（ME）从21种克罗地亚植物中分离出游离挥发性化合物，并采用气相色谱 - 质谱联用进行分析。主成分分析（PCA）根据主要化合物的相对比例区分出一些聚类，如十六烷酸、六氢法呢基丙酮、叶绿醇、β-石竹烯和石竹烯氧化物，这两种分离方法研究的所有物种中均鉴定出了这些化合物。除这些化合物外，在采用ME分离的来自干旱生境的五个样品中还鉴定出了吉马烯D、δ-芹子烯和二十烷。在通过两种方法分离的来自潮湿生境的五个物种中，α-香橙烯和β-紫罗兰酮特别丰富。采用HD分离的来自温和生境的物种的特性是苯乙醛、壬醛以及从烃类中鉴定出的重要化合物，而ME的特性是（E）-β-大马酮。在本文中，我们展示了来自不同生境植物的植物化学特征的新结果。这些化合物的生物潜力应进一步研究，以便更好地理解和利用这些特殊的植物代谢产物。
