Nazlić Marija, Akrap Karla, Kremer Dario, Dunkić Valerija
Faculty of Science, University of Split, Ruđera Boškovića 33, HR-21000 Split, Croatia.
Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, Croatia.
Pharmaceuticals (Basel). 2022 Nov 9;15(11):1378. doi: 10.3390/ph15111378.
In this study, free volatile compounds (FVCs) were isolated from the water fractions (hydrosols) of 10 Croatian species obtained by hydrodistillation (HD) and microwave-assisted extraction (MAE). The use of different isolation techniques is important for the analysis of the influence of extraction conditions on the qualitative and quantitative composition of the isolated constituents. The composition of the hydrosols was analyzed using gas chromatography and mass spectrometry. The compounds -ionone and benzene acetaldehyde were detected in all 10 hydrosols studied. -caryophyllene was also identified in all isolates except the MAE isolate of L. Caryophyllene oxide was isolated in all isolates apart from the HD isolate of Pennell. (-Damascenone is significantly present in all isolates except the MAE isolates of and Bodard. In these two species, -muurolol was identified in a high percentage. The same basic phytochemical constituents and compounds characteristic of a given species suggest the importance of further research. Antioxidant activity was tested for all extracts using two methods, ORAC and DPPH. Therefore, it is crucial to identify as many specialized metabolites from species as possible, especially hydrosols, which are natural products of potential pharmacological interest.
在本研究中，从通过水蒸馏（HD）和微波辅助萃取（MAE）获得的10种克罗地亚植物的水相馏分（纯露）中分离出游离挥发性化合物（FVCs）。使用不同的分离技术对于分析萃取条件对分离成分的定性和定量组成的影响很重要。使用气相色谱和质谱法分析纯露的组成。在所研究的所有10种纯露中均检测到了化合物紫罗兰酮和苯乙醛。除了唇形科植物的MAE分离物外，在所有分离物中也鉴定出了β-石竹烯。除了佩内尔氏植物的HD分离物外，在所有分离物中均分离出了石竹烯氧化物。在除了某植物和博达德氏植物的MAE分离物之外的所有分离物中，（-大马酮均大量存在。在这两个物种中，鉴定出了高比例的β-桉叶醇。相同的基本植物化学成分和特定物种特有的化合物表明了进一步研究的重要性。使用ORAC和DPPH两种方法对所有提取物的抗氧化活性进行了测试。因此，尽可能多地从植物物种中鉴定出专门的代谢物至关重要，尤其是纯露，它们是具有潜在药理学意义的天然产物。
