Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Aleje Raclawickie 1, 20-059 Lublin, Poland.
Molecules. 2022 Apr 6;27(7):2345. doi: 10.3390/molecules27072345.
Leaves of L., L., L. and L. are considered agro-waste of the berry industry, but they can be a rich source of valuable bioactive compounds used in cosmetic industry. In this study, kombucha-fermented and non-fermented extracts were compared in terms of chemical composition and biological activity. Polyphenol compounds were identified by HPLC/DAD/ESI-MS. The antioxidant potential was analyzed by evaluating the scavenging of intracellular free radicals contained in keratinocytes and fibroblasts and by DPPH and ABTS assay, obtaining a higher radical scavenging capacity for the ferments, especially for and ferments. Assessment of the cytotoxicity on skin cell lines showed their positive effect on the viability of fibroblasts and keratinocytes (especially for the ferments after 10 days of fermentation). The potential anti-ageing properties were determined by their ability to inhibit the activity of metalloproteinases, obtaining almost 30% inhibition of collagenase and elastase in the case of fermented . Moreover, when the samples were applied to the skin, the positive effect of ferments on skin hydration and pH was demonstrated, which indicates that kombucha berry leaf extracts may be an innovative cosmetic ingredient.
、、、的叶子被认为是浆果产业的农业废料，但它们可能是一种有价值的生物活性化合物的丰富来源，可用于化妆品行业。在这项研究中，比较了康普茶发酵和未发酵提取物在化学成分和生物活性方面的差异。通过 HPLC/DAD/ESI-MS 鉴定多酚化合物。通过评估角质细胞和成纤维细胞内自由基的清除率以及 DPPH 和 ABTS 测定来分析抗氧化潜力，发酵提取物（特别是 和 发酵提取物）具有更高的自由基清除能力。对皮肤细胞系的细胞毒性评估表明，它们对成纤维细胞和角质细胞的活力有积极影响（特别是发酵 10 天后的发酵提取物）。通过抑制金属蛋白酶的活性来确定其潜在的抗老化特性，发酵提取物对胶原蛋白酶和弹性蛋白酶的抑制率接近 30%。此外，当将这些样品应用于皮肤时，证明了发酵提取物对皮肤水合作用和 pH 值的积极影响，这表明康普茶浆果叶提取物可能是一种创新的化妆品成分。
