AMOREPACIFIC Research and Innovation Center, Yongin, Republic of Korea.
Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea.
Skin Res Technol. 2023 Jun;29(6):e13354. doi: 10.1111/srt.13354.
Wrinkles represent a characteristic symptom of skin aging. In recent years, various studies have focused on their prevention and/or cure. However, clinical tests are still the only method available to directly detect and evaluate the anti-wrinkle efficacy of various substances. Moreover, no in vitro strategy for such anti-aging skin analysis has been reported. Therefore, in this study, we aimed to develop a novel technology to overcome these limitations.
Full-thickness (FT) skin wrinkle mimics with various widths and depths were fabricated using a collagen stamping method. These were analyzed and compared using 2D and 3D Swept Source-Optical Coherence Tomography (SS-OCT) imaging technologies.
SS-OCT demonstrated superficial and cross-sectional images of the wrinkle mimics, and the size of the wrinkles was validated using image analysis. Retinoic acid treatment significantly decreased both the depth and width of wrinkles formed in the FT skin wrinkle mimics.
Using 3D tissue engineering and SS-OCT imaging technologies, we developed a novel in vitro technique that can directly detect skin wrinkles. This significantly efficient method could lead to an alternative strategy for animal experiments and preclinical anti-aging research on the skin.
皱纹是皮肤衰老的特征性症状。近年来，人们对其预防和/或治疗方法进行了各种研究。然而，临床测试仍然是直接检测和评估各种物质抗皱效果的唯一方法。此外，尚无用于此类抗衰老皮肤分析的体外策略。因此，在本研究中，我们旨在开发一种新技术来克服这些局限性。
使用胶原冲压法制备具有不同宽度和深度的全厚（FT）皮肤皱纹模拟物。使用 2D 和 3D 扫频源光学相干断层扫描（SS-OCT）成像技术对其进行分析和比较。
SS-OCT 显示了皱纹模拟物的浅层和横截面图像，并且使用图像分析验证了皱纹的大小。维甲酸处理可显著减小 FT 皮肤皱纹模拟物中形成的皱纹的深度和宽度。
我们使用 3D 组织工程和 SS-OCT 成像技术开发了一种新颖的体外技术，可以直接检测皮肤皱纹。这种高效的方法可以替代动物实验和皮肤抗衰老研究的临床前策略。
