Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005324.
A novel bacterial strain, designated MK52, was isolated from the phycosphere of . Strain MK52 is a Gram-stain-negative, pink-pigmented, rod-shaped, strictly aerobic bacterium. In 16S rRNA phylogenetic analysis, the MK52 strain was most closely related to RW1 (98.66 %) and UM2 (98.51 %). The genomic DNA G+C content of strain MK52 was calculated to be 65.5 mol%. The average nucleotide identity values of strain MK52 with RW1 and UM2 were 80.35 and 80.23 %, respectively, with digital DNA-DNA hybridization values of 23.6 and 22.9 %, respectively, and average amino acid identities of 75.59 and 75.79 %, respectively. The major isoprenoid quinone was Q-10, and the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid. Fatty acid methyl ether analysis showed that summed feature 8 (C ω7 and/or C ω6) was the main cellular fatty acid in strain MK52. Strain MK52 cells grew at 21-34 °C (optimum, 30 °C), pH 5-8 (optimum, pH 7) and with 0-2 % (w/v) NaCl (optimum, 0.5 % NaCl). sp. nov. is proposed as a new species (=KCTC 72877=DSM 111424) based on its genotypic and phenotypic characteristics.
一株新的细菌菌株，命名为 MK52，从. 的藻际中分离出来。菌株 MK52 是革兰氏阴性、粉红色、杆状、严格需氧菌。在 16S rRNA 系统发育分析中，MK52 菌株与 RW1（98.66%）和 UM2（98.51%）最为密切相关。菌株 MK52 的基因组 DNA G+C 含量为 65.5 mol%。MK52 菌株与 RW1 和 UM2 的平均核苷酸同一性值分别为 80.35%和 80.23%，数字 DNA-DNA 杂交值分别为 23.6%和 22.9%，平均氨基酸同一性值分别为 75.59%和 75.79%。主要的异戊二烯醌是 Q-10，主要的极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和神经酰胺糖脂。脂肪酸甲酯醚分析表明，特征脂肪酸 8（C ω7 和/或 C ω6）是菌株 MK52 的主要细胞脂肪酸。菌株 MK52 细胞在 21-34°C（最佳温度为 30°C）、pH 5-8（最佳 pH 为 7）和 0-2%（w/v）NaCl（最佳为 0.5% NaCl）下生长。根据其基因型和表型特征，提议将. 种作为一个新种（=KCTC 72877=DSM 111424）。
