Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, Thailand.
Laboratory of Veterinary teaching hospital of Mahasarakham University, Maha Sarakham 44000, Thailand.
J Vet Sci. 2022 Sep;23(5):e73. doi: 10.4142/jvs.22111. Epub 2022 Aug 9.
Lumpy skin disease (LSD), a disease transmitted by direct and indirect contact with infected cattle, is caused by the Lumpy skin disease virus (LSDV). The disease affects cattle herds in Africa, Europe, and Asia. The clinical signs of LSD range from mild to the appearance of nodules and lesions in the skin leading to severe symptoms that are sometimes fatal with significant livestock economic losses.
This study aimed to characterize LSDV strains in the blood of infected cattle in Thailand based on the GPCR gene and determine the phylogenetic relationship of LSDV Thailand isolates with published sequences available in the database.
In total, the blood samples of 120 cattle were collected from different farms in four provinces in the northeastern part of Thailand, and the occurrence of LSDV was examined by PCR based on the P32 antigen gene. The genetic diversity of LSDV based on the GPCR gene was analyzed.
Polymerase chain reaction assays based on the P32 antigen gene showed that 4.17% (5/120) were positive for LSDV. All positive blood samples were amplified successfully for the GPCR gene. Phylogenetic analysis showed that LSDV Thailand isolates clustered together with LSDVs from China and Russia.
The LSD outbreak in Thailand was confirmed, and a phylogenetic tree was constructed to infer the branching pattern of the GPCR gene from the presence of LSDV in Thailand. This is the first report on the molecular characterization of LSDV in cattle in Thailand.
块状皮肤病（LSD）是一种由与受感染牛直接或间接接触传播的疾病，由块状皮肤病病毒（LSDV）引起。该病影响非洲、欧洲和亚洲的牛群。LSD 的临床症状从轻微到皮肤出现结节和病变，导致严重症状，有时甚至致命，并给畜牧业造成重大经济损失。
本研究旨在根据 GPCR 基因对泰国感染牛血液中的 LSDV 株进行特征描述，并确定与数据库中已发表序列相比，泰国 LSDV 分离株的系统进化关系。
从泰国东北部四个省的不同农场共采集了 120 份牛血样，通过基于 P32 抗原基因的 PCR 检测 LSDV 的发生情况。分析了基于 GPCR 基因的 LSDV 的遗传多样性。
基于 P32 抗原基因的聚合酶链反应检测显示，4.17%（5/120）的 LSDV 为阳性。所有阳性血液样本均成功扩增出 GPCR 基因。系统进化分析表明，泰国 LSDV 分离株与来自中国和俄罗斯的 LSDV 聚集在一起。
证实了泰国的 LSD 爆发，并构建了一个系统进化树，以推断泰国 LSDV 存在时 GPCR 基因的分支模式。这是泰国牛块状皮肤病病毒分子特征的首次报告。
