Dobros Natalia, Zawada Katarzyna, Paradowska Katarzyna
Department of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha Str. 1, 02-097 Warsaw, Poland.
Antioxidants (Basel). 2022 Apr 5;11(4):711. doi: 10.3390/antiox11040711.
Lavender is a valuable perennial plant from the Lamiaceae family. It is grown mainly for its essential oil, but it also contains polar bioactive compounds such as polyphenols and coumarins. Their level depends on the species, cultivars, geographical origin, climatic conditions, harvest time and extraction method. The authors investigated the effect of several extraction procedures (maceration, decoction and ultrasound-assisted extraction) applied to three cultivars of (Betty's Blue, Elizabeth, Hidcote) and two cultivars of x (Grosso, Gros Bleu) on the yield of the polyphenolic compounds and antioxidant activity. HPLC analysis showed the presence of rosmarinic acid (2.52-10.82 mg/g), ferulic acid glucoside (2.94-8.67 mg/g), caffeic acid (1.70-3.10 mg/g), morin (1.02-13.63 mg/g), coumarin (1.01-5.97 mg/g) and herniarin (1.05-8.02 mg/g). The content of phenolic acids and flavonoids was higher in lavender, while the content of coumarins was higher in lavandin in all types of extracts. The antioxidant activity was determined by DPPH-EPR assay for antiradical properties (104.58-206.77 μmol Trolox/g) and FRAP assay for reducing properties (79.21-203.06 μmol Trolox/g). The obtained results showed that the cultivar is the dominant factor differentiating the samples. Still, the extraction method plays an important role in the final bioactive substances content and antioxidant properties of obtained extracts.
薰衣草是唇形科一种有价值的多年生植物。它主要因其精油而种植,但也含有极性生物活性化合物,如多酚和香豆素。它们的含量取决于品种、栽培品种、地理来源、气候条件、收获时间和提取方法。作者研究了几种提取方法(浸渍法、煎煮法和超声辅助提取法)对三种薰衣草品种(贝蒂蓝、伊丽莎白、希德科特)和两种醒目薰衣草品种(格罗索、格罗斯蓝)中多酚类化合物产量和抗氧化活性的影响。高效液相色谱分析表明存在迷迭香酸(2.52 - 10.82毫克/克)、阿魏酸葡萄糖苷(2.94 - 8.67毫克/克)、咖啡酸(1.70 - 3.10毫克/克)、桑色素(1.02 - 13.63毫克/克)、香豆素(1.01 - 5.97毫克/克)和蛇床子素(1.05 - 8.02毫克/克)。在所有类型的提取物中,薰衣草中酚酸和黄酮类化合物的含量较高,而醒目薰衣草中香豆素的含量较高。通过DPPH - EPR法测定抗自由基性能的抗氧化活性(104.58 - 206.77微摩尔 Trolox/克),通过FRAP法测定还原性能的抗氧化活性(79.21 - 203.06微摩尔 Trolox/克)。所得结果表明,品种是区分样品的主导因素。然而,提取方法对所得提取物中最终生物活性物质的含量和抗氧化性能起着重要作用。