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有效高通量分离富含血小板和循环促血管生成细胞以加速皮肤伤口愈合。

Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing.

机构信息

Novystem Spa, viale Piave 21, 20129, Milan, Italy.

Unit of Neurology, Stem Cell Laboratory, Department of Pathophysiology and Transplantation, Universitá degli Studi di Milano, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, via Francesco Sforza 35, 20122, Milan, Italy.

出版信息

Cell Mol Life Sci. 2022 Apr 26;79(5):259. doi: 10.1007/s00018-022-04284-4.

DOI:10.1007/s00018-022-04284-4
PMID:35474498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9042989/
Abstract

Delayed wound healing and chronic skin lesions represent a major health problem. Over the past years, growth factors mediated by platelet-rich plasma (PRP) and cell-based therapies were developed as effective and affordable treatment able to improve wound healing capacity. We have advanced existing concepts to develop a highly efficient high-throughput protocol with proven application for the isolation of PRP and pro-angiogenic cells (Angio). This protocol outlines the effectiveness of Angio in promoting the critical healing process including wound closure, re-epithelialization, granulation tissue growth, and blood vessel regeneration. We coupled this effect with normalization of mechanical properties of rescued mouse wounds, which is sustained by a correct arrangement of elastin and collagen fibers. Proteomic analysis of treated wounds demonstrated a fingerprint of Angio based on the up-regulation of detoxification pathway of glutathione metabolism, correlated to a decrease in inflammatory response. Overall, these results have enabled us to provide a framework for how Angio supports wound healing, opening avenues for further clinical advances.

摘要

延迟性伤口愈合和慢性皮肤损伤是一个主要的健康问题。在过去的几年中，血小板丰富的血浆（PRP）和基于细胞的治疗所介导的生长因子被开发为有效且负担得起的治疗方法，能够提高伤口愈合能力。我们已经改进了现有概念，开发了一种高效的高通量方案，该方案已被证明可用于 PRP 和促血管生成细胞（Angio）的分离。该方案概述了 Angio 在促进关键愈合过程中的有效性，包括伤口闭合、再上皮化、肉芽组织生长和血管再生。我们将这种效果与恢复的小鼠伤口机械性能的正常化相结合，这是通过弹性蛋白和胶原蛋白纤维的正确排列来维持的。经处理的伤口的蛋白质组学分析显示了基于谷胱甘肽代谢解毒途径上调的 Angio 的特征，这与炎症反应的减少相关。总的来说，这些结果使我们能够提供 Angio 如何支持伤口愈合的框架，为进一步的临床进展开辟了途径。

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