Zhang Xin, Zhang Yaping, Gui Xinru, Zhang Yanli, Zhang Zhenhong, Chen Wendan, Zhang Xiaowei, Wang Yanxiang, Zhang Mengjiao, Shang Ziqi, Xin Yiwei, Zhang Yi
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China.
Molecular Diagnostics Center, Qilu Hospital of Shandong University, Jinan 250012, China.
iScience. 2022 Apr 4;25(5):104203. doi: 10.1016/j.isci.2022.104203. eCollection 2022 May 20.
(Fn) is primarily colonized in the oral cavity. Recently, Fn has been closely associated with the tumorigenesis of colorectal cancer (CRC). Here, we showed that the relative level of Fn DNA was increased in the saliva of the CRC group compared with the normal colonoscopy, hyperplastic polyp, and adenoma groups. Receiver operating characteristic curve analysis illustrated that Fn DNA was superior to carcinoembryonic antigen and carbohydrate antigen 19-9 in CRC diagnosis. Moreover, levels of Fn DNA were associated with the overall survival and disease-free survival of CRC patients, which was an independent factor for prognostic prediction. Transcriptome sequencing identified 1,287 differentially expressed mRNAs in tumor tissues between CRC patients with high-Fn and low-Fn infection. Kyoto encyclopedia of genes and genomes analysis showed that ECM-receptor interaction and focal adhesion were the top two significant pathways. Overall, salivary Fn DNA may be a noninvasive diagnostic and prognostic biomarker for CRC patients.
具核梭杆菌（Fn）主要定植于口腔。最近，Fn与结直肠癌（CRC）的肿瘤发生密切相关。在此，我们表明，与正常结肠镜检查、增生性息肉和腺瘤组相比，CRC组唾液中Fn DNA的相对水平升高。受试者工作特征曲线分析表明，Fn DNA在CRC诊断中优于癌胚抗原和糖类抗原19-9。此外，Fn DNA水平与CRC患者的总生存期和无病生存期相关，这是预后预测的独立因素。转录组测序确定了高Fn感染和低Fn感染的CRC患者肿瘤组织中1287个差异表达的mRNA。京都基因与基因组百科全书分析表明，细胞外基质-受体相互作用和粘着斑是前两个显著的通路。总体而言，唾液Fn DNA可能是CRC患者的一种非侵入性诊断和预后生物标志物。
