一种释放一氧化氮的葡聚糖衍生物的抗血栓形成特性：血小板活化和全血凝血动力学评估
Anti-thrombogenic properties of a nitric oxide-releasing dextran derivative: evaluation of platelet activation and whole blood clotting kinetics.
作者信息
Damodaran Vinod B, Leszczak Victoria, Wold Kathryn A, Lantvit Sarah M, Popat Ketul C, Reynolds Melissa M
机构信息
Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
Department of Mechanical Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO-80523, USA.
出版信息
RSC Adv. 2013 Dec 14;3(46). doi: 10.1039/C3RA45521A.
Abstract
Controlling platelet activation and clotting initiated by cardiovascular interventions remains a major challenge in clinical practice. In this work, the anti-thrombotic properties of a polysaccharide-based nitric oxide (NO)-releasing dextran derivative are presented. Total platelet adhesion, platelet morphology and whole blood clotting kinetics were used as indicators to evaluate the anti-clotting properties of this material. With a total NO delivery of 0.203±0.003 μmol, the NO-releasing dextran derivative (Dex-SNO) mixed with blood plasma demonstrated a significantly lower amount of platelet adhesion and activation onto a surface and reduced whole blood clotting kinetics. Nearly 75% reduction in platelet adhesion and a significant retention of platelet morphology were observed with blood plasma treated with Dex-SNO, suggesting this to be a potential anti-platelet therapeutic agent for preventing thrombosis that does not have an adverse effect on circulating platelets.
摘要
控制心血管介入引发的血小板活化和凝血在临床实践中仍然是一项重大挑战。在这项工作中，展示了一种基于多糖的一氧化氮（NO）释放葡聚糖衍生物的抗血栓特性。使用总血小板粘附、血小板形态和全血凝血动力学作为指标来评估该材料的抗凝血特性。在总NO释放量为0.203±0.003 μmol的情况下，与血浆混合的NO释放葡聚糖衍生物（Dex-SNO）在表面上表现出显著更低的血小板粘附和活化量，并降低了全血凝血动力学。用Dex-SNO处理的血浆观察到血小板粘附减少了近75%，并且血小板形态得到了显著保留，这表明它是一种潜在的抗血小板治疗剂，可用于预防血栓形成，且对循环血小板没有不良影响。
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