用于测量和操纵代谢的基因编码工具。
Genetically encoded tools for measuring and manipulating metabolism.
机构信息
Department of Nutritional Sciences & Toxicology, University of California, Berkeley, CA, USA.
Center for Computational Biology, University of California, Berkeley, CA, USA.
出版信息
Nat Chem Biol. 2022 May;18(5):451-460. doi: 10.1038/s41589-022-01012-8. Epub 2022 Apr 28.
Abstract
Over the past few years, we have seen an explosion of novel genetically encoded tools for measuring and manipulating metabolism in live cells and animals. Here, we will review the genetically encoded tools that are available, describe how these tools can be used and outline areas where future development is needed in this fast-paced field. We will focus on tools for direct measurement and manipulation of metabolites. Metabolites are master regulators of metabolism and physiology through their action on metabolic enzymes, signaling enzymes, ion channels and transcription factors, among others. We hope that this Perspective will encourage more people to use these novel reagents or even join this exciting new field to develop novel tools for measuring and manipulating metabolism.
摘要
在过去的几年中，我们看到了用于测量和操纵活细胞和动物代谢的新型遗传编码工具的激增。在这里，我们将回顾现有的遗传编码工具，描述如何使用这些工具，并概述在这个快速发展的领域中需要进一步发展的领域。我们将重点介绍用于直接测量和操纵代谢物的工具。代谢物通过作用于代谢酶、信号酶、离子通道和转录因子等，成为代谢和生理学的主要调节剂。我们希望本观点能鼓励更多的人使用这些新型试剂，甚至加入这个令人兴奋的新领域，开发用于测量和操纵代谢的新型工具。
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