疟疾血清学中确定的恶性疟原虫抗原
Defined Plasmodium falciparum antigens in malaria serology.
作者信息
Gabra M S, Grossiord D, Perrin L H, Shaw A, Cheung A, McGregor I A
出版信息
Bull World Health Organ. 1986;64(6):889-96.
Abstract
The study evaluates three enzyme-linked immunosorbent assays (ELISA) of malaria antigens suitable for use in large-scale epidemiological studies. Results obtained using sera from 567 persons from the Gambia indicated that the micro-ELISA method using parasitized red blood cell extract did not reliably quantitate antimalarial antibodies, especially in young children. In contrast, two micro-ELISA methods that employed purified, defined antigens (a polypeptide of M(r) = 41 000 present in rhoptries, and a 31-1 fusion polypeptide corresponding to a merozoite surface antigen) permitted the precise determination of antimalarial antibodies in both adults and children. Problems and advantages associated with the use of the M(r) = 41 000 and 31-1 antigens for the determination of antimalarial antibodies are discussed.
摘要
该研究评估了三种适用于大规模流行病学研究的疟疾抗原酶联免疫吸附测定（ELISA）方法。使用来自冈比亚的567人的血清所获得的结果表明，使用寄生红细胞提取物的微量ELISA方法不能可靠地定量抗疟抗体，尤其是在幼儿中。相比之下，两种使用纯化的特定抗原（存在于棒状体中的分子量为41 000的多肽，以及对应于裂殖子表面抗原的31-1融合多肽）的微量ELISA方法能够精确测定成人和儿童体内的抗疟抗体。文中讨论了使用分子量为41 000的抗原和31-1抗原测定抗疟抗体的相关问题和优点。
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