Liu Guoyan, Chu Meng, Xu Piao, Nie Shiying, Xu Xin, Ren Jiaoyan
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Food Chem X. 2022 Jan 4;13:100206. doi: 10.1016/j.fochx.2022.100206. eCollection 2022 Mar 30.
The effects of aquatic proteins on the proliferation and adhesion of intestinal probiotic bacteria were investigated by in vitro fermentation and mouse in vitrointestinal tissue models. Compared with the control group, the protein reduced the growth time of Lactobacillus plantarum (LP45) by 34.25% and increased the total number of colonies by 6.61%. The salt-solubale protein performed better than water-soluble protein in vitro proliferation of LP45. salt-soluble protein significantly increased the number of viable bacteria adhering to intestinal, and caused changes in the amount of polysaccharides, proteins and biofilms in the intestinal tissue model. These results indicate that the protein is beneficial to the proliferation and adhesion of probiotics in the intestinal, and can be used as an active protein beneficial to intestinal health.
通过体外发酵和小鼠体外肠道组织模型研究了水产蛋白对肠道益生菌增殖和黏附的影响。与对照组相比,该蛋白使植物乳杆菌(LP45)的生长时间缩短了34.25%,菌落总数增加了6.61%。盐溶性蛋白在LP45的体外增殖方面比水溶性蛋白表现更好。盐溶性蛋白显著增加了黏附于肠道的活菌数量,并导致肠道组织模型中多糖、蛋白质和生物膜量的变化。这些结果表明,该蛋白有利于益生菌在肠道内的增殖和黏附,可作为一种有益于肠道健康的活性蛋白。