Li Hui, Pang Yang, Wang Xin, Cao Xun, He Xun, Chen Kequan, Li Ganlu, Ouyang Pingkai, Tan Weiming
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University Nanjing 210000 China
State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing 210000 China.
RSC Adv. 2019 Feb 25;9(12):6548-6555. doi: 10.1039/c8ra09827a. eCollection 2019 Feb 22.
Methods for enhancing enzyme activities in two-phase systems are getting more attention. Phospholipase D (PLD) was successfully encapsulated into metal-surfactant nanocapsules (MSNCs) using a one-pot self-assembly technique in an aqueous solution. The highest yield for the production of high-value phosphatidylserine (PS) from low-value phosphatidylcholine (PC) in the two-phase system was achieved by encapsulating PLD into MSNCs formed from Ca which gave an enzyme activity that was 133.6% of that of free PLD. The PLD@MSNC transformed the two-phase system into an emulsion phase system and improved the organic solvent tolerance, pH and thermal stabilities as well as the storage stability and reusability of the enzyme. Under optimal conditions, PLD@MSNC generated 91.9% PS over 8 h in the two-phase system, while free PLD generated only 77.5%.
提高两相系统中酶活性的方法正受到越来越多的关注。利用一锅自组装技术在水溶液中将磷脂酶D（PLD）成功封装到金属表面活性剂纳米胶囊（MSNCs）中。通过将PLD封装到由钙形成的MSNCs中，在两相系统中从低价值的磷脂酰胆碱（PC）生产高价值的磷脂酰丝氨酸（PS）时获得了最高产率，其酶活性为游离PLD的133.6%。PLD@MSNC将两相系统转变为乳液相系统，并提高了酶的有机溶剂耐受性、pH稳定性、热稳定性以及储存稳定性和可重复使用性。在最佳条件下，PLD@MSNC在两相系统中8小时内产生了91.9%的PS，而游离PLD仅产生了77.5%。
