Fendler Cornelius, Harberts Jann, Rafeldt Lars, Loers Gabriele, Zierold Robert, Blick Robert H
Center for Hybrid Nanostructures (CHyN), Universität Hamburg 22761 Hamburg Germany
Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf (UKE) Hamburg 20251 Germany.
Nanoscale Adv. 2020 Jul 14;2(11):5192-5200. doi: 10.1039/d0na00549e. eCollection 2020 Nov 11.
Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and grooves serve as cell culture platforms. The effects of contact guidance through topography and chemical guidance through selective poly-d-lysine coating on these platforms are analyzed. As a consequence, the herein presented fabrication approach can be utilized to cultivate and to study low-density neuronal networks in 2.5D configuration with a high degree of order.
神经突的定向引导是细胞与半导体组件之间界面定制设计的先决条件。灰度光刻、反应离子蚀刻和紫外纳米压印光刻是与半导体工业兼容的有效技术，可用于经济高效地制造调制表面。在这项工作中，研究了用这些方法制备的2.5D路径上小鼠小脑神经元的神经突生长情况。微米级台阶和沟槽结构用作细胞培养平台。分析了通过形貌的接触引导和通过选择性聚-d-赖氨酸涂层的化学引导对这些平台的影响。因此，本文提出的制造方法可用于培养和研究具有高度有序性的2.5D配置的低密度神经网络。
