Lin Zixin, Tong Yingpeng, Li Na, Zhu Ziping, Li Junmin
School of Life Science, Shanghai Normal University Shanghai 200234 China.
Zhejiang Provincial Key Laboratory of Evolutionary Ecology and Conservation, Taizhou University Taizhou 318000 China
RSC Adv. 2020 Oct 7;10(61):37168-37181. doi: 10.1039/d0ra06846b.
Diabetes is a complex illness requiring long-term therapy. , a recently confirmed new food resource, shows significant hypoglycemic and hypolipidemic effects in type II diabetes. Triterpenoid saponins are considered as the effective medicinal components of and are useful for the treatment of diabetes mellitus. However, little is known regarding their specific mechanism of actions. In this study, we used active ingredient screening and target prediction techniques to determine the components of responsible for its anti-diabetic effects as well as their targets. In addition, we used bioinformatics technology and molecular docking analysis to determine the mechanisms underlying their anti-diabetic effects. A total of 39 triterpenes were identified through a literature search and 1 triterpene compound by experiments. In all, 33 potential target proteins associated with 36 pathways were predicted to be related to diabetes. Finally, 7 compounds, 15 target proteins, and 15 signaling pathways were found to play important roles in the therapeutic effects of against diabetes. These results provide a theoretical framework for the use of against diabetes. Moreover, molecular docking verification showed that more than 90% of the active ingredients had binding activity when tested against key target proteins, and a literature search showed that the active ingredients identified had anti-diabetic effects, indicating that the results were highly reliable.
糖尿病是一种需要长期治疗的复杂疾病。[具体食物资源名称未给出]作为一种最近确认的新食物资源，在II型糖尿病中显示出显著的降血糖和降血脂作用。三萜皂苷被认为是[具体食物资源名称未给出]的有效药用成分，对糖尿病的治疗有用。然而，关于它们的具体作用机制知之甚少。在本研究中，我们使用活性成分筛选和靶点预测技术来确定[具体食物资源名称未给出]中负责其抗糖尿病作用的成分及其靶点。此外，我们使用生物信息学技术和分子对接分析来确定其抗糖尿病作用的潜在机制。通过文献检索共鉴定出39种三萜，通过实验鉴定出1种三萜化合物。总共预测了与36条途径相关的33种潜在靶蛋白与糖尿病有关。最后，发现7种化合物、15种靶蛋白和15条信号通路在[具体食物资源名称未给出]对糖尿病的治疗作用中起重要作用。这些结果为使用[具体食物资源名称未给出]治疗糖尿病提供了理论框架。此外，分子对接验证表明，超过90%的活性成分在针对关键靶蛋白进行测试时具有结合活性，文献检索表明所鉴定的活性成分具有抗糖尿病作用，表明结果高度可靠。
