Stem Cell Medicine and Stem Cell and Organoid Facility, University of Sydney, Westmead, 2145 NSW, Australia.
Stem Cell Medicine and Stem Cell and Organoid Facility, University of Sydney, Westmead, 2145 NSW, Australia; Synapse Proteomics, University of Sydney, Westmead, 2145 NSW, Australia.
Stem Cell Reports. 2022 Jun 14;17(6):1476-1492. doi: 10.1016/j.stemcr.2022.04.003. Epub 2022 May 5.
Advances in the study of neurological conditions have been possible because of pluripotent stem cell technologies and organoids. Studies have described the generation of neural ectoderm-derived retinal and brain structures from pluripotent stem cells. However, the field is still troubled by technical challenges, including high culture costs and variability. Here, we describe a simple and economical protocol that reproducibly gives rise to the neural retina and cortical brain regions from confluent cultures of stem cells. The spontaneously generated cortical organoids are transcriptionally comparable with organoids generated by other methods. Furthermore, these organoids showed spontaneous functional network activity and proteomic analysis confirmed organoids maturity. The generation of retinal and brain organoids in close proximity enabled their mutual isolation. Suspension culture of this complex organoid system demonstrated the formation of nerve-like structures connecting retinal and brain organoids, which might facilitate the investigation of neurological diseases of the eye and brain.
神经疾病研究的进展得益于多能干细胞技术和类器官。研究已经描述了从多能干细胞生成神经外胚层衍生的视网膜和脑结构。然而，该领域仍然受到技术挑战的困扰，包括高培养成本和变异性。在这里，我们描述了一种简单经济的方案，可以从干细胞的汇合培养物中重现地产生神经视网膜和皮质脑区。自发产生的皮质类器官在转录上与其他方法生成的类器官相当。此外，这些类器官表现出自发的功能网络活动，蛋白质组学分析证实了类器官的成熟。视网膜和脑类器官的近距离生成使得它们能够相互分离。这种复杂的类器官系统的悬浮培养显示出连接视网膜和脑类器官的神经样结构的形成，这可能有助于研究眼睛和大脑的神经疾病。
