College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
Fujian Key Laboratory of Advanced Technology for Cancer Screening and Early Diagnosis，Fujian Cancer Hospital， Fuzhou, China.
Int J Biol Macromol. 2022 Jun 15;210:377-393. doi: 10.1016/j.ijbiomac.2022.05.008. Epub 2022 May 6.
Clinically, systemic antibiotic therapy and traditional dressings care are not satisfactory in treating chronic diabetic ulcers (DU). Therefore, we presented sprayable antibacterial hydrogel for effective treatment of DU by using antibacterial macromolecules (quaternized chitosan, QCS, Mn ≈ 1.5 × 10), photothermal antibacterial nanoparticles (ε-poly-l-lysine grafted graphene quantum dots, GQDs-ε-PL) and miocompatible macromolecules (benzaldehyde-terminated four-arm poly(ethylene glycol), 4 arm PEG-BA) as materials. The results revealed that the hydrogel could be in situ formed in 70-89 s through dynamic imine bonds crosslinking and exhibited a pH-dependent swelling ability and degradability. The hydrogel could respond to bacterial triggered acidic environment to play a synergistic effect of chemotherapy and xenon light irradiated PTT, leading to the rupture of the bacterial membrane and the inactivation of bacteria, promoting the migration and proliferation of fibroblast cell, enhancing the adhesion of platelet endothelial cell, and finally accelerating the healing of infected diabetic wound. Moreover, the hydrogel displayed self-healing, hemostatic, and biocompatible abilities, which could provide a better healing environment for wound and further promote wound healing. Hence, the multifunctional hydrogel is expected to be a potential dressing for the clinical treatment of DU.
临床上，全身抗生素治疗和传统敷料护理在治疗慢性糖尿病溃疡（DU）方面并不令人满意。因此，我们提出了一种可喷涂的抗菌水凝胶，通过使用抗菌大分子（季铵化壳聚糖，QCS，Mn≈1.5×10）、光热抗菌纳米粒子（ε-聚-l-赖氨酸接枝石墨烯量子点，GQDs-ε-PL）和生物相容性大分子（苯甲醛封端的四臂聚乙二醇，4 臂 PEG-BA）作为材料来有效治疗 DU。结果表明，水凝胶可通过动态亚胺键交联在 70-89 s 内原位形成，并表现出 pH 依赖性溶胀能力和可降解性。水凝胶可响应细菌触发的酸性环境，发挥化学治疗和氙光照射 PTT 的协同作用，导致细菌膜破裂和失活，促进成纤维细胞的迁移和增殖，增强血小板内皮细胞的黏附，并最终加速感染性糖尿病伤口的愈合。此外，水凝胶还具有自修复、止血和生物相容性能力，可为伤口提供更好的愈合环境，进一步促进伤口愈合。因此，这种多功能水凝胶有望成为治疗 DU 的潜在敷料。
