Richards R L, Hayre M D, Hockmeyer W T, Alving C R
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
Infect Immun. 1988 Mar;56(3):682-6. doi: 10.1128/iai.56.3.682-686.1988.
A liposome-encapsulated cloned protein (R32tet32) containing sequences from the tetrapeptide repeat region of the circumsporozoite protein of Plasmodium falciparum sporozoites was examined for immunogenicity with rabbits and monkeys. Effects of adjuvants were tested by encapsulation of the antigen in liposomes either lacking or containing lipid A and adsorption with aluminum hydroxide (ALUM). When rabbits were immunized with R32tet32 alone, a primary antibody response was not seen and a secondary response did not appear until 32 to 36 weeks after boosting. Immunization with ALUM-adsorbed R32tet32 resulted in a minimal primary antibody response. A moderate secondary antibody response was detected within 2 weeks after boosting, but antibody levels decreased to preimmunization levels 8 weeks after boosting. When R32tet32 was encapsulated in liposomes containing lipid A, strong primary and secondary antibody responses were observed. Strong primary and secondary responses also were obtained when R32tet32 was encapsulated in liposomes either containing or lacking lipid A and the liposomes were adsorbed with ALUM. The strongest antibody response was obtained by immunization with ALUM-adsorbed liposomes containing lipid A and R32tet32, suggesting that the adjuvant effects of liposomes, lipid A, and ALUM were additive or synergistic.
对一种脂质体包裹的克隆蛋白（R32tet32）进行了研究，该蛋白含有恶性疟原虫子孢子环子孢子蛋白四肽重复区域的序列，用兔子和猴子检测其免疫原性。通过将抗原包裹在不含或含有脂多糖A的脂质体中以及用氢氧化铝（ALUM）吸附来测试佐剂的效果。当单独用R32tet32免疫兔子时，未观察到初次抗体反应，直到加强免疫后32至36周才出现二次反应。用ALUM吸附的R32tet32免疫产生的初次抗体反应极小。加强免疫后2周内检测到中等程度的二次抗体反应，但加强免疫8周后抗体水平降至免疫前水平。当R32tet32包裹在含有脂多糖A的脂质体中时，观察到强烈的初次和二次抗体反应。当R32tet32包裹在含或不含脂多糖A的脂质体中且脂质体用ALUM吸附时，也获得了强烈的初次和二次反应。通过用含脂多糖A和R32tet32的ALUM吸附脂质体免疫获得了最强的抗体反应，表明脂质体、脂多糖A和ALUM的佐剂作用是相加或协同的。
