Muhammad Wali, Ullah Naimat, Haroon Muhammad, Abbasi Bilal Haider
Department of Biotechnology, Quaid-i-Azam University 45320 Islamabad Pakistan
Department of Chemistry, Quaid-i-Azam University 45320 Islamabad Pakistan.
RSC Adv. 2019 Sep 18;9(51):29541-29548. doi: 10.1039/c9ra04424h.
Biogenic synthesis using medicinal plants has less harmful effects as compared to chemically synthesized nanoparticles. Here, for the first time, we successfully demonstrated the eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) using an aqueous extract of L. The phyto-mediated ZnO NPs were characterized using UV-visible spectroscopy, XRD (X-ray diffraction), FT-IR (Fourier transform infrared spectroscopy), SEM (scanning electron microscopy) and TEM (transmission electron microscopy). They were also evaluated for anti-diabetic activity, biocompatibility with RBCs and bactericidal biological applications. The UV spectrum showed a strong surface plasmon peak for ZnO NPs at 360 nm. The optical band gap was observed to be 2.93 eV using UV spectroscopy data. The crystalline nature and the crystal size (48 nm) of the prepared ZnO NPs were confirmed by XRD. FT-IR analysis confirmed the formation of functional bio-molecules linked with ZnO NPs. SEM and TEM images revealed irregular and spherical morphology. The ZnO NPs demonstrated moderate enzyme inhibition (30.8%) at a concentration of 200 mg ml. No potential damage was caused by ZnO NPs to red blood cells, if used in low doses. aqueous extract has the potency to combat drug-resistant bacteria but comparatively, ZnO NPs synthesized from the same plant were found to be more effective against resistant pathogenic strains. It is concluded from the above study that phyto-fabricated ZnO NPs have strong potential as theranostic agents and can be adopted in drug delivery systems.
与化学合成的纳米颗粒相比，利用药用植物进行生物合成具有较小的有害影响。在此，我们首次成功展示了使用L.的水提取物对氧化锌纳米颗粒（ZnO NPs）进行生态友好型合成。利用紫外可见光谱、XRD（X射线衍射）、FT-IR（傅里叶变换红外光谱）、SEM（扫描电子显微镜）和TEM（透射电子显微镜）对植物介导的ZnO NPs进行了表征。还对它们的抗糖尿病活性、与红细胞的生物相容性以及杀菌生物应用进行了评估。紫外光谱显示ZnO NPs在360 nm处有一个很强的表面等离子体峰。利用紫外光谱数据观察到光学带隙为2.93 eV。通过XRD确认了所制备的ZnO NPs的晶体性质和晶体尺寸（48 nm）。FT-IR分析证实了与ZnO NPs相连的功能性生物分子的形成。SEM和TEM图像显示出不规则和球形的形态。ZnO NPs在浓度为200 mg/ml时表现出中等程度的酶抑制作用（30.8%）。如果低剂量使用，ZnO NPs不会对红细胞造成潜在损害。水提取物有对抗耐药细菌的潜力，但相比之下，从同一植物合成的ZnO NPs对耐药致病菌株更有效。从上述研究可以得出结论，植物制备的ZnO NPs作为治疗诊断剂具有很强的潜力，可用于药物递送系统。
