三种用于从鼻腔冲洗样本中检测呼吸道合胞病毒的快速诊断技术的比较。
Comparison of three rapid diagnostic techniques for detection of respiratory syncytial virus from nasal wash specimens.
作者信息
Chonmaitree T, Bessette-Henderson B J, Hepler R E, Lucia H L
出版信息
J Clin Microbiol. 1987 Apr;25(4):746-7. doi: 10.1128/jcm.25.4.746-747.1987.
Abstract
We report results of three rapid tests for respiratory syncytial virus antigen detection. An immunofluorescence assay using commercial antibody and two commercial enzyme immunoassays (Ortho Diagnostics, Inc., Raritan, N.J., and Abbott Laboratories, North Chicago, Ill.) were applied to 199 nasal wash specimens. The Abbott enzyme immunoassay was the most sensitive technique, with a sensitivity of 93.8%. The specificities of the three techniques were comparable and greater than 95%. The availability of reliable rapid diagnostic techniques will allow for better care of infants with severe respiratory syncytial virus infection.
摘要
我们报告了三种呼吸道合胞病毒抗原检测快速试验的结果。使用商业抗体的免疫荧光测定法以及两种商业酶免疫测定法（新泽西州拉里坦市的奥索诊断公司和伊利诺伊州北芝加哥的雅培实验室）应用于199份鼻洗液标本。雅培酶免疫测定法是最敏感的技术，灵敏度为93.8%。这三种技术的特异性相当，均大于95%。可靠的快速诊断技术的可用性将有助于更好地护理患有严重呼吸道合胞病毒感染的婴儿。
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本文引用的文献
1
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J Clin Microbiol. 1982 Aug;16(2):329-33. doi: 10.1128/jcm.16.2.329-333.1982.
2
3
Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study.雾化吸入利巴韦林治疗呼吸道合胞病毒感染婴儿。一项随机双盲研究。
N Engl J Med. 1983 Jun 16;308(24):1443-7. doi: 10.1056/NEJM198306163082403.
4
Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture.呼吸道合胞病毒酶联免疫吸附测定与HEp-2细胞培养的比较
J Clin Microbiol. 1985 Dec;22(6):1071-2. doi: 10.1128/jcm.22.6.1071-1072.1985.
5
6
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J Clin Microbiol. 1985 Nov;22(5):782-5. doi: 10.1128/jcm.22.5.782-785.1985.
7
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J Med Virol. 1986 May;19(1):1-9. doi: 10.1002/jmv.1890190102.
8
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J Clin Microbiol. 1986 Mar;23(3):485-8. doi: 10.1128/jcm.23.3.485-488.1986.
9
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