McIntosh K, Hendry R M, Fahnestock M L, Pierik L T
J Clin Microbiol. 1982 Aug;16(2):329-33. doi: 10.1128/jcm.16.2.329-333.1982.
An enzyme-linked immunosorbent assay (ELISA) for respiratory syncytial virus antigens was applied to the rapid diagnosis of acute infections in children and was compared with viral culture and immunofluorescence tests. The ELISA test employed commercially available reagents and was run on a day-to-day basis as specimens were received in the laboratory. Sensitivity and specificity by ELISA were 82 and 95%, respectively, compared with culture. In the same specimens, the sensitivity and specificity by immunofluorescence were 86 and 96%, respectively. Nasopharyngeal aspirates were proven to be a better source of viral antigen than were nasopharyngeal swabs. ELISA-positive samples remained positive even when left unrefrigerated for a week or mailed to the laboratory in plastic containers. Respiratory syncytial virus ELISA, like culture, became negative as the disease progressed and showed no superiority over culture for diagnosis late in the illness.
一种用于检测呼吸道合胞病毒抗原的酶联免疫吸附测定（ELISA）方法被应用于儿童急性感染的快速诊断，并与病毒培养和免疫荧光检测进行了比较。ELISA检测使用市售试剂，每天在实验室收到标本时进行检测。与病毒培养相比，ELISA的灵敏度和特异性分别为82%和95%。在相同标本中，免疫荧光的灵敏度和特异性分别为86%和96%。事实证明，鼻咽抽吸物是比鼻咽拭子更好的病毒抗原来源。ELISA阳性样本即使在未冷藏一周或用塑料容器邮寄到实验室的情况下仍保持阳性。呼吸道合胞病毒ELISA与病毒培养一样，随着疾病进展会转为阴性，在疾病后期诊断方面并不比病毒培养更具优势。
