呼吸道合胞病毒酶联免疫吸附测定与HEp-2细胞培养的比较
Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture.
作者信息
Bromberg K, Tannis G, Daidone B, Clarke L, Sierra M F
出版信息
J Clin Microbiol. 1985 Dec;22(6):1071-2. doi: 10.1128/jcm.22.6.1071-1072.1985.
Abstract
Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. A blocking test confirmed those specimens as true positives (specificity, 100%).
摘要
采用美国新泽西州拉里坦市奥索诊断公司的呼吸道合胞病毒抗原酶联免疫吸附试验，对270份鼻咽抽吸物进行了重复检测。与细胞培养法相比，该检测方法的敏感性为80%至82%，特异性为96%。酶联免疫吸附试验在细胞培养中呼吸道合胞病毒呈阳性的71份标本之外，又检测出7份抗原阳性标本。一项阻断试验证实这些标本为真阳性（特异性为100%）。
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J Virol Methods. 1981 Oct;3(3):137-47. doi: 10.1016/0166-0934(81)90048-3.
2
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3
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N Engl J Med. 1983 Jun 16;308(24):1443-7. doi: 10.1056/NEJM198306163082403.
4
Nosocomial viral infections: I. Epidemiology and significance.医院内病毒感染：I. 流行病学及意义
Infect Control. 1980 Jan-Feb;1(1):33-7. doi: 10.1017/s0195941700052371.
5
Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique.应用免疫荧光技术对儿童呼吸道合胞病毒感染进行快速诊断。
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6
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N Engl J Med. 1973 Mar 8;288(10):498-505. doi: 10.1056/NEJM197303082881005.
7
Clinically useful method for the isolation of respiratory syncytial virus.分离呼吸道合胞病毒的临床实用方法。
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