Jiao Yan, Li Dajing, Liu Chunquan, Chang Ying, Song Jiangfeng, Xiao Yadong
Institute of Farm Products Processing, Jiangsu Academy of Agricultural Sciences Nanjing 210014 China
College of Food and Biological Engineering, Qiqihar University Qiqihar 161006 China.
RSC Adv. 2018 Sep 6;8(55):31372-31381. doi: 10.1039/c8ra05838e. eCollection 2018 Sep 5.
Lutein (LUT) is a bioactive food compound found in various vegetables and plays a critical role in the promotion of health and well-being. However, lutein is an unstable molecule which has a very low bioavailability caused by its poor solubility in aqueous media, and is poorly absorbed when administered orally. To enhance the stability, release and bioactivity of lutein, poly-l-lysine (PLL) decorated nanoliposomes (PLL-LUT-NLP) were developed as novel delivery systems for lutein. The mean particle size of PLL-LUT-NLP was found to be in the range 264-367 nm with a low polydispersity index (PDI < 0.4). The zeta potential changed from -38.6 mV in undecorated nanoliposomes to -27.9 mV in PLL-decorated nanoliposomes. Furthermore, the lutein entrapment efficiency (EE%) of PLL-LUT-NLP was found to be highest in nanoliposomes decorated with 0.06% (w/v) PLL. PLL could protect lutein in nanoliposomes from degradation and promote the lutein release from the nanoliposomes in gastrointestinal fluid conditions. Additionally, the PLL-decorated nanoliposomes maintained the antioxidant activity of the lutein, and the antiproliferative activity was more significant than that of undecorated nanoliposomes in inhibiting the proliferation of human tumor cells. These results suggest that PLL-decorated nanoliposomes have potential to be used for efficient delivery of lutein and further improve its bioavailability.
叶黄素（LUT）是一种存在于多种蔬菜中的生物活性食品化合物，在促进健康和幸福方面起着关键作用。然而，叶黄素是一种不稳定的分子，由于其在水性介质中的溶解度差，生物利用度非常低，口服时吸收也很差。为了提高叶黄素的稳定性、释放度和生物活性，开发了聚-L-赖氨酸（PLL）修饰的纳米脂质体（PLL-LUT-NLP）作为叶黄素的新型递送系统。发现PLL-LUT-NLP的平均粒径在264-367nm范围内，多分散指数较低（PDI<0.4）。zeta电位从未修饰的纳米脂质体中的-38.6mV变为PLL修饰的纳米脂质体中的-27.9mV。此外，发现用0.06%（w/v）PLL修饰的纳米脂质体中PLL-LUT-NLP的叶黄素包封率（EE%）最高。PLL可以保护纳米脂质体中的叶黄素不被降解，并促进叶黄素在胃肠液条件下从纳米脂质体中释放。此外，PLL修饰的纳米脂质体保持了叶黄素的抗氧化活性，并且在抑制人肿瘤细胞增殖方面，其抗增殖活性比未修饰的纳米脂质体更显著。这些结果表明，PLL修饰的纳米脂质体有潜力用于高效递送叶黄素并进一步提高其生物利用度。
