评价一种含有提取物的凝胶在体内创伤愈合模型中的作用。

Evaluation of a Gel Containing a Extract in an In Vivo Model of Wound Healing.

机构信息

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31-98166 Messina, Italy.

Unit of General Surgery and Surgical Oncology, Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy.

出版信息

Int J Mol Sci. 2022 Apr 24;23(9):4708. doi: 10.3390/ijms23094708.

DOI:10.3390/ijms23094708

PMID:35563099

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9101165/

Abstract

Inappropriate wound healing (WH) management can cause significant comorbidities, especially in patients affected by chronic and metabolic diseases, such as diabetes. WH involves several different, partially overlapping processes, including hemostasis, inflammation, cell proliferation, and remodeling. Oxidative stress in WH contributes to WH impairment because of the overexpression of radical oxygen species (ROS) and nitrogen species (RNS). This study aimed to evaluate the in vitro antioxidative action of a gel containing a extract (Emorsan Gel) and assess its skin re-epithelialization properties in a mouse model of WH. The scavenging effects of the bacterial extract were assessed in vitro through the ABTS and DPPH assays and in L-929 murine fibroblasts. The effects of the Emorsan Gel were studied in vivo in a murine model of WH. After WH induction, mice were treated daily with vehicle or Emorsan Gel for 6 or 12 days. According to the in vitro tests, the extract exerted an inhibitory effect on ROS and RNS, consequently leading to the reduction in malondialdehyde (MDA) and nitrite levels. Before proceeding with the in vivo study, the Emorsan Gel was verified to be unabsorbed. Therefore, the observed effects could be ascribed to a local action. The results obtained in vivo showed that through local reduction of oxidative stress and inflammation (IL-1β, TNF-α), the Emorsan Gel significantly reduced the infiltration of mast cells into the injured wound, leading to the amelioration of symptoms such as itch and skin irritation. Therefore, the Emorsan Gel improved the speed and percentage of wound area closure by improving the tissue remodeling process, prompting vascular-endothelial growth factor (VEGF) and transforming growth factor (TGF)- β production and reducing the expression of adhesion molecules. Emorsan Gel, by its ability to inhibit free radicals, could reduce local inflammation and oxidative stress, thus enhancing the speed of wound healing.

摘要

（不合理的）伤口愈合（WH）管理会导致严重的合并症，特别是在患有慢性和代谢性疾病（如糖尿病）的患者中。WH 涉及几个不同的、部分重叠的过程，包括止血、炎症、细胞增殖和重塑。WH 中的氧化应激会导致 WH 受损，因为自由基（ROS）和氮自由基（RNS）的过度表达。本研究旨在评估一种含有提取物（Emorsan Gel）的凝胶的体外抗氧化作用，并评估其在 WH 小鼠模型中的皮肤再上皮化特性。通过 ABTS 和 DPPH 测定法和 L-929 鼠成纤维细胞评估细菌提取物的清除作用。在 WH 诱导后，将小鼠每天用载体或 Emorsan Gel 处理 6 或 12 天。根据体外试验，提取物对 ROS 和 RNS 有抑制作用，从而导致丙二醛（MDA）和亚硝酸盐水平降低。在进行体内研究之前，验证了 Emorsan Gel 不被吸收。因此，观察到的效果可能归因于局部作用。体内研究结果表明，通过局部减少氧化应激和炎症（IL-1β，TNF-α），Emorsan Gel 显著减少了损伤伤口中肥大细胞的浸润，从而改善了瘙痒和皮肤刺激等症状。因此，Emorsan Gel 通过改善组织重塑过程，促进血管内皮生长因子（VEGF）和转化生长因子（TGF）-β的产生，减少粘附分子的表达，从而提高了伤口面积闭合的速度和百分比。Emorsan Gel 通过抑制自由基的能力，可以减轻局部炎症和氧化应激，从而加快伤口愈合速度。