Gameta Health Centre, 7 Cybernetyki St., 02-677 Warsaw, Poland.
Gameta, Kielce-Regional Science-Technology Centre, 45 Podzamcze St., Chęciny, 26-060 Kielce, Poland.
Int J Environ Res Public Health. 2022 Apr 22;19(9):5117. doi: 10.3390/ijerph19095117.
Pyrethroids exposure has been associated with adverse reproductive outcome. However, there is no study that explores the effect of environmental exposure and embryological outcomes. This question was addressed in a prospective cohort of couples undergoing fertility treatment. The study aims to assess the association between urinary metabolites of synthetic pyrethroids and embryological outcomes (MII oocyte count, top quality embryo, fertilization and implantation rate). We included 450 women aged 25−45 undergoing assisted reproductive technology (ART) cycle at Infertility Clinic in Poland. Urine samples were collected at the time of fertility procedure(s) to assess four urinary synthetic pyrethroids concentrations (3-phenoxybenzoic acid (3PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (cis-DCCA), trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (trans-DCCA), cis-2,2-dibromovinyl-2,2-dimethylocyclopropane-1-carboxylic acid (DBCA)) using validated gas chromatography ion-tap mass spectrometry and calculated for each cycle-specific metabolite. To evaluate the effect of environmental exposure to synthetic pyrethroids and embryological outcomes (methaphase II (MII) oocyte yield, top quality embryo, fertilization rate, implantation rate), multivariable generalized linear mixed analyses with random intercepts were prepared. Urinary 3-PBA concentrations decrease MII oocyte count (p = 0.007) in the fourth quartile (>75 percentile) compared to women in the first quartile (≤25 percentile). Additionally, when 3-PBA was treated as continuous variable, the negative association between exposure to pyrethroids and MII oocyte count was also observed (p = 0.012). Exposure to other pyrethroid metabolities (CDCCA, TDCCA, DBCA) was not related to any of the examined embryological outcomes. Exposure to synthetic pyrethroids may be associated with poorer embryological outcome among couples seeking fertility treatments. As this is the first study on this topic, the results need to be confirmed in further studies.
拟除虫菊酯暴露与不良生殖结局有关。然而，目前尚无研究探讨环境暴露与胚胎发育结局之间的关系。本研究通过前瞻性队列研究，调查了接受辅助生殖技术（ART）治疗的夫妇的尿液代谢物与胚胎发育结局（MII 卵母细胞计数、优质胚胎、受精率和着床率）之间的关系。我们纳入了 450 名年龄在 25-45 岁之间的波兰不孕症诊所接受辅助生殖技术（ART）周期的妇女。在进行生育程序时采集尿液样本，以评估四种尿液中合成拟除虫菊酯的浓度（3-苯氧基苯甲酸（3PBA）、顺式-3-（2,2-二氯乙烯基）-2,2-二甲基环丙烷羧酸（cis-DCCA）、反式-3-（2,2-二氯乙烯基）-2,2-二甲基环丙烷羧酸（trans-DCCA）、顺式-2,2-二溴乙烯基-2,2-二甲基环丙烷-1-羧酸（DBCA）），使用经过验证的气相色谱离子阱质谱法进行测定，并计算每个周期特异性代谢物的浓度。为了评估环境暴露于合成拟除虫菊酯与胚胎发育结局（MII 卵母细胞产量、优质胚胎、受精率、着床率）之间的关系，我们采用了具有随机截距的多变量广义线性混合分析。与第一四分位数（≤25 百分位数）的女性相比，第四四分位数（>75 百分位数）的尿液 3-PBA 浓度降低了 MII 卵母细胞计数（p = 0.007）。此外，当 3-PBA 作为连续变量时，也观察到拟除虫菊酯暴露与 MII 卵母细胞计数之间存在负相关（p = 0.012）。其他拟除虫菊酯代谢产物（CDCCA、TDCCA、DBCA）的暴露与任何检查的胚胎发育结局均无关。在寻求生育治疗的夫妇中，合成拟除虫菊酯的暴露可能与较差的胚胎发育结局有关。由于这是该主题的第一项研究，因此需要在进一步的研究中证实这些结果。
