Xie Chengjia, Zhang Qun, Li Zhao, Ge Shaohua, Ma Baojin
Department of Periodontology & Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Shandong University, Jinan 250012, China.
Polymers (Basel). 2022 Apr 29;14(9):1816. doi: 10.3390/polym14091816.
During wound healing, bacterial infection is one of the main limiting factors for the desired efficiency. Wound dressing-mediated antibiotics therapies could overcome this problem to a great extent due to sustained drug release and controllable dose. Here, we designed a kind of alginate injectable hydrogel loaded with minocycline (SA@MC) as a dressing for staphylococcus aureus-infected wound healing. SA@MC hydrogel possessed good injectability and can be injected by syringes. MC participated in the gel formation, causing the microstructure change based on the morphology characterization. The element mapping and FT-IR spectra further confirmed the successful loading of MC in SA hydrogel. Interestingly, MC was released more efficiently in a weakly alkaline condition (pH 7-8) than in a weakly acidic condition (pH 4-6) from SA@MC injectable hydrogel, which means that there is an accelerated release to respond to the weakly alkaline wound microenvironment. Meanwhile, SA@MC injectable hydrogel had high biocompatibility and excellent antibacterial activity due to the sustained release of MC. Further, in vivo experiment results demonstrated that SA@MC injectable hydrogel promoted staphylococcus aureus-infected wound healing efficiently. In summary, the injectable composite hydrogel can serve as an ideal dressing to prevent bacterial infection and promote wound healing.
在伤口愈合过程中，细菌感染是影响预期疗效的主要限制因素之一。伤口敷料介导的抗生素疗法由于药物持续释放和剂量可控，在很大程度上可以克服这一问题。在此，我们设计了一种负载米诺环素的海藻酸盐可注射水凝胶（SA@MC）作为金黄色葡萄球菌感染伤口愈合的敷料。SA@MC水凝胶具有良好的可注射性，可通过注射器注射。米诺环素参与凝胶形成，根据形态学表征导致微观结构发生变化。元素映射和傅里叶变换红外光谱进一步证实了米诺环素成功负载于海藻酸钠水凝胶中。有趣的是，米诺环素从SA@MC可注射水凝胶在弱碱性条件（pH 7 - 8）下比在弱酸性条件（pH 4 - 6）下释放更有效，这意味着对弱碱性伤口微环境有加速释放响应。同时，由于米诺环素的持续释放，SA@MC可注射水凝胶具有高生物相容性和优异的抗菌活性。此外，体内实验结果表明，SA@MC可注射水凝胶能有效促进金黄色葡萄球菌感染伤口的愈合。综上所述，这种可注射复合水凝胶可作为预防细菌感染和促进伤口愈合的理想敷料。
