Liu Yuguang, Jeraldo Patricio, Herbert William, McDonough Samantha, Eckloff Bruce, Schulze-Makuch Dirk, de Vera Jean-Pierre, Cockell Charles, Leya Thomas, Baqué Mickael, Jen Jin, Walther-Antonio Marina
Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, MN 55905, USA.
Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA.
iScience. 2022 Apr 25;25(5):104291. doi: 10.1016/j.isci.2022.104291. eCollection 2022 May 20.
The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving under extreme conditions and thus is of great interest for the astrobiology community. The knowledge of its complete genome sequence would serve as a guide for further studies. However, a major concern has been placed on the effects of contamination on the quality of sequencing data without a reference genome. Here, we report the use of microfluidic technology combined with single cell sequencing and assembly to minimize the contamination and recover the complete genome of the Nostoc strain CCCryo 231-06 with high quality. 100% of the whole genome was recovered with all contaminants removed and a strongly supported phylogenetic tree. The data reported can be useful for comparative genomics for phylogenetic and taxonomic studies. The method used in this work can be applied to studies that require high-quality assemblies of genomes of unknown microorganisms.
念珠藻属菌株CCCryo 231-06是一种能够在极端条件下生存的蓝藻菌株，因此受到天体生物学界的极大关注。其完整基因组序列的知识将为进一步研究提供指导。然而，在没有参考基因组的情况下，污染对测序数据质量的影响一直是一个主要问题。在这里，我们报告了使用微流控技术结合单细胞测序和组装，以尽量减少污染并高质量地恢复念珠藻菌株CCCryo 231-06的完整基因组。去除了所有污染物并构建了一个支持度很高的系统发育树，100%的全基因组得以恢复。所报告的数据可用于系统发育和分类研究的比较基因组学。这项工作中使用的方法可应用于需要对未知微生物基因组进行高质量组装的研究。
