Suzuki Yuki, Kawamata Ibuki, Watanabe Kotaro, Mano Eriko
Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8578, Japan.
Department of Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
iScience. 2022 Apr 25;25(5):104292. doi: 10.1016/j.isci.2022.104292. eCollection 2022 May 20.
The DNA origami technique is used to construct custom-shaped nanostructures that can be used as components of two-dimensional crystalline structures with user-defined structural patterns. Here, we designed an Mg-responsive hexagonal 3D DNA origami block with self-shape-complementary ruggedness on the sides. Hexagonal DNA origami blocks were electrostatically adsorbed onto a fluidic lipid bilayer membrane surface to ensure lateral diffusion. A subsequent increase in the Mg concentration in the surrounding environment induced the self-assembly of the origami blocks into lattices with prescribed geometries based on a self-complementary shape fit. High-speed atomic force microscopy (HS-AFM) images revealed dynamic events involved in the self-assembly process, including edge reorganization, defect splitting, diffusion, and filling, which provide a glimpse into how the lattice structures are self-improved.
DNA折纸技术用于构建定制形状的纳米结构，这些结构可用作用户定义结构图案的二维晶体结构的组件。在这里，我们设计了一种对镁有响应的六边形3D DNA折纸块，其侧面具有自形状互补的凹凸不平。六边形DNA折纸块通过静电吸附到流体脂质双分子层膜表面，以确保横向扩散。随后，周围环境中镁浓度的增加促使折纸块基于自互补形状匹配自组装成具有规定几何形状的晶格。高速原子力显微镜（HS-AFM）图像揭示了自组装过程中涉及的动态事件，包括边缘重组、缺陷分裂、扩散和填充，这让我们得以一窥晶格结构是如何自我完善的。
