State Key Laboratory of High-Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of High-Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Biomater Adv. 2022 Mar;134:112699. doi: 10.1016/j.msec.2022.112699. Epub 2022 Feb 6.
Implant infection, undesirable inflammation, and poor osseointegration are the primary reasons for implant failure, so it is pivotal to endow bone implants with antibacterial, anti-inflammatory, and osteogenic properties. Here, a multifunctional fluorine-doped zirconium-metal organic framework (Zr-MOF) film was constructed on the titanium to modify its biological performances. The fumaric acid, a common antioxidant, was selected as the ligand of Zr-MOF, and the hydrofluoric acid was used as the modulator to control the growth of Zr-MOF film. The obtained fluorine-doped Zr-MOF film possessed good biocompatibility and osteogenic ability, and it showed good antibacterial effects against both gram-positive S. aureus and gram-negative E. coli due to the release of fluoride ions. In addition, the doping of fluorine could reduce the stability of Zr-MOF by substituting fumaric acid, and stimulating the releases of fumaric acid. Furthermore, the fumaric acid released from Zr-MOF could down-regulate the expression of pro-inflammatory genes (NF-κB and IL-6), but up-regulate the expression of anti-inflammatory gene of IL-4 of macrophage, showing good anti-inflammatory ability. This study provided a reference for the modulation synthesis of MOF film, and proposed a promising strategy of designing Zr-MOF film to endow bone implants with antibacterial, anti-inflammatory, and osteogenic abilities.
植入物感染、不良炎症和骨整合不良是植入物失败的主要原因，因此赋予骨植入物抗菌、抗炎和成骨特性至关重要。在这里，构建了一种多功能氟掺杂锆金属有机骨架（Zr-MOF）薄膜，以修饰钛的生物性能。富马酸是一种常见的抗氧化剂，被选为 Zr-MOF 的配体，而氢氟酸被用作调节剂来控制 Zr-MOF 薄膜的生长。所获得的氟掺杂 Zr-MOF 薄膜具有良好的生物相容性和成骨能力，由于氟离子的释放，对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌都具有良好的抗菌效果。此外，氟的掺杂通过取代富马酸和刺激富马酸的释放来降低 Zr-MOF 的稳定性。此外，Zr-MOF 释放的富马酸可以下调促炎基因（NF-κB 和 IL-6）的表达，但上调巨噬细胞抗炎基因 IL-4 的表达，表现出良好的抗炎能力。本研究为 MOF 薄膜的调制合成提供了参考，并提出了一种有前途的设计 Zr-MOF 薄膜的策略，赋予骨植入物抗菌、抗炎和成骨能力。
