Fernando Ilekuttige Priyan Shanura, Kirindage Kirinde Gedara Isuru Sandanuwan, Jayasinghe Arachchige Maheshika Kumari, Han Eui Jeong, Dias Mawalle Kankanamge Hasitha Madhawa, Kang Kyung Pil, Moon Sung Ig, Shin Tai Sun, Ma Ayeong, Jung Kyungsook, Ahn Ginnae
Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea.
Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea.
Antioxidants (Basel). 2022 May 20;11(5):1013. doi: 10.3390/antiox11051013.
(Hack.) Makino & Shibata or broad-leaf bamboo is famous for its richness of bioactive natural products and its uses in traditional medicine for its anti-inflammatory, diuretic, and antipyretic properties and preventive effects against hypertension, arteriosclerosis, cardiovascular disease, and cancer. The present study investigated the antioxidant activity of hot water extract (SBH) and its effects in ameliorating hydrogen peroxide-induced oxidative stress, using an African green monkey kidney epithelial cell line (Vero). Known polyphenols in SBH were quantified by HPLC analysis. SBH indicated a dose-dependent increase for reducing power, ABTS (IC = 96.44 ± 0.61 µg/mL) and DPPH (IC = 125.78 ± 4.41 µg/mL) radical scavenging activities. SBH markedly reduced intracellular reactive oxygen species (ROS) generation in the Vero cells and increased the protective effects against HO-induced oxidative stress by reducing apoptosis. Other than the direct involvement in neutralizing ROS, metabolites in SBH were also found to induce NRF2-mediated production of antioxidant enzymes, HO-1, and NQO1. These findings imply that hot water extract can be utilized to create nutraceutical and functional foods that can help to relieve the effects of oxidative stress in both acute and chronic kidney injury.
（哈克。）牧野和柴田或阔叶竹以其丰富的生物活性天然产物以及在传统医学中的应用而闻名，具有抗炎、利尿、解热特性以及对高血压、动脉硬化、心血管疾病和癌症的预防作用。本研究使用非洲绿猴肾上皮细胞系（Vero）研究了热水提取物（SBH）的抗氧化活性及其改善过氧化氢诱导的氧化应激的作用。通过高效液相色谱分析对SBH中已知的多酚进行了定量。SBH的还原能力、ABTS（IC = 96.44 ± 0.61 µg/mL）和DPPH（IC = 125.78 ± 4.41 µg/mL）自由基清除活性呈剂量依赖性增加。SBH显著降低了Vero细胞内活性氧（ROS）的产生，并通过减少细胞凋亡增强了对HO诱导的氧化应激的保护作用。除了直接参与中和ROS外，还发现SBH中的代谢产物可诱导NRF2介导的抗氧化酶HO-1和NQO1的产生。这些发现表明，热水提取物可用于制造营养保健品和功能性食品，有助于缓解急性和慢性肾损伤中的氧化应激影响。
