Siotto Mariacristina, Germanotta Marco, Santoro Massimo, Canali Raffaella, Pascali Simona, Insalaco Sabina, Cipollini Valeria, Papadopoulou Dionysia, Antonacci Erika, Aprile Irene
IRCCS Fondazione Don Carlo Gnocchi ONLUS, 50143 Florence, Italy.
Energy and Sustainable Economic Development, Division of Health Protection Technologies ENEA-Italian National Agency for New Technologies, 00123 Rome, Italy.
Healthcare (Basel). 2022 May 9;10(5):869. doi: 10.3390/healthcare10050869.
After a cerebral stroke insult, there is an overproduction of Reactive Oxygen Species (ROS), which overcome the antioxidant defenses, causing further tissues damage. The status of oxidative stress in stroke patients over time, particularly in those undergoing rehabilitation treatments, has been poorly investigated. We analyzed the oxidative stress status in 61 subacute stroke patients (33 females and 28 males) admitted to our rehabilitation center by measuring, in serum: hydroperoxides levels (d-ROMs), antioxidant activity (BAP test), and the relative antioxidant capacity (OSI index). We also analyzed patients for glucose levels and lipid profile. In addition, we analyzed the correlation between oxidative stress status biomarkers and motor deficits, disability, and pain. Almost all patients showed high or very high levels of d-ROMs, while BAP levels were apparently in the reference range of normality. Females had lower BAP values (females: 2478 ± 379; males: 2765 ± 590; = 0.034) and lower OSI index (females: 5.7 ± 1.9; males: 6.8 ± 1.9; = 0.043). Moreover, in the male group, the correlation with motor impairment and disability showed a worsened motor performance when oxidative stress is higher. Female group, on the other hand, had an unexpected different trend of correlation, probably due to an unbalanced systemic oxidative stress. Further research is needed to see if sex differences in oxidative stress status in subacute stroke patients persist after rehabilitation.
脑卒中新发损伤后，活性氧（ROS）生成过量，超出抗氧化防御能力，导致进一步的组织损伤。卒中患者氧化应激状态随时间的变化情况，尤其是接受康复治疗的患者，尚未得到充分研究。我们通过检测血清中的氢过氧化物水平（d-ROMs）、抗氧化活性（BAP检测）和相对抗氧化能力（OSI指数），分析了61例入住我们康复中心的亚急性卒中患者（33例女性和28例男性）的氧化应激状态。我们还分析了患者的血糖水平和血脂情况。此外，我们分析了氧化应激状态生物标志物与运动功能障碍、残疾和疼痛之间的相关性。几乎所有患者的d-ROMs水平都很高或非常高，而BAP水平明显在正常参考范围内。女性的BAP值较低（女性：2478±379；男性：2765±590；P = 0.034），OSI指数也较低（女性：5.7±1.9；男性：6.8±1.9；P = 0.043）。此外，在男性组中，氧化应激越高，与运动障碍和残疾的相关性显示运动表现越差。另一方面，女性组的相关性趋势意外不同，可能是由于全身氧化应激不平衡所致。需要进一步研究以确定亚急性卒中患者氧化应激状态的性别差异在康复后是否持续存在。
