Department of Dermatology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy.
Institute of Biomedical Technologies, National Research Council, 20054 Segrate, Italy.
Int J Mol Sci. 2022 May 17;23(10):5625. doi: 10.3390/ijms23105625.
(CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic.
Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., "CT-negative" ( = 21) and "CT-positive" ( = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by H-NMR.
Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by , and being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family-in particular, of the species-was observed during CT infection. The genus was positively correlated with urine hippurate and lactulose.
These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches.
（CT）是全球最常见的细菌性性传播感染的病原体。到目前为止，关于 CT 尿道炎患者首次晨尿样本中的微生物组成，相关信息还很少。因此，在这项研究中，我们对性传播感染门诊就诊的 CT 尿道感染女性的首次晨尿样本进行了微生物组和代谢组学特征分析。
根据尿液样本中核酸扩增技术检测 CT 的阳性结果，将入组的女性分为两组，即“CT 阴性”（=21）和“CT 阳性”（=11）。尿液样本用于：（i）通过 16s rRNA 基因测序进行微生物组分析，（ii）通过 H-NMR 进行代谢组分析。
无论是否存在 CT 感染，首次晨尿的微生物组主要由 、 和 主导，其中 是最具代表性的物种。与对照组相比，CT 阳性样本的微生物多样性减少。此外，在 CT 感染期间，观察到支原体科家族（尤其是 种）显著减少。 属与尿 hippurate 和 lactulose 呈正相关。
这些数据有助于阐明衣原体泌尿生殖道感染的发病机制，并为创新的诊断和治疗方法奠定基础。
