Tokajuk Joanna, Deptuła Piotr, Chmielewska Sylwia J, Skłodowski Karol, Mierzejewska Żaneta A, Grądzka-Dahlke Małgorzata, Tołstoj Adam, Daniluk Tamara, Paprocka Paulina, Savage Paul B, Bucki Robert
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland.
Dentistry and Medicine Tokajuk, Żelazna 9/7, 15-297 Bialystok, Poland.
Pathogens. 2022 Apr 20;11(5):491. doi: 10.3390/pathogens11050491.
Recurrent oral infections, as manifested by endodontic and periodontal disease, are often caused by () and (). Here, we assessed the anti-biofilm activity of ceragenin CSA-44 against these microbes growing as a biofilm in the presence of saliva on the surface of human teeth and dental composite (composite filling) subjected to mechanical stresses. Biofilm mass analysis was performed using crystal violet (CV) staining. The morphology, viscoelastic properties of the biofilm after CSA-44 treatment, and changes in the surface of the composite in response to biofilm presence were determined by AFM microscopy. CSA-44 prevented biofilm formation and reduced the mass of biofilm formed by tested microorganisms on teeth and dental composite. The ability of CSA-44 to prevent the formation and to reduce the presence of established biofilm on tooth and composite filling suggests that it can serve as an agent in the development of new methods of combating oral pathogens and reduce the severity of oral infections.
复发性口腔感染，如牙髓病和牙周病所表现的那样，通常由（）和（）引起。在此，我们评估了鲨胺素CSA - 44对在人牙齿表面以及承受机械应力的牙科复合材料（复合填充物）上以生物膜形式在唾液存在的情况下生长的这些微生物的抗生物膜活性。使用结晶紫（CV）染色进行生物膜质量分析。通过原子力显微镜（AFM）确定CSA - 44处理后生物膜的形态、粘弹性特性以及复合材料表面因生物膜存在而发生的变化。CSA - 44可防止生物膜形成，并减少受试微生物在牙齿和牙科复合材料上形成的生物膜质量。CSA - 44防止生物膜形成以及减少牙齿和复合填充物上已形成生物膜的存在的能力表明，它可作为开发对抗口腔病原体新方法的一种药剂，并降低口腔感染的严重程度。
