Shen Xin, Yang Yutao, Li Jian, Zhang Bo, Wei Wei, Lu Changqing, Yan Caixia, Wei Hong, Li Yan
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Institute of Immunology, PLA, Army Medical University, Chongqing 400038, China.
Pathogens. 2022 Apr 26;11(5):513. doi: 10.3390/pathogens11050513.
The immune dysregulation induced by periodontal bacteria has important roles in the development of periodontitis. However, the role of key periodontal bacteria in local and systemic immunity has not been comprehensively studied. Herein, to explore immunoregulation maps of key periodontal bacteria, a mono-colonized germ-free mice model with , , and for two weeks was designed in this study. The alveolar bone loss was determined by micro-CT. A total of 14 types of innate and adaptive immune cells of the gingiva, spleen, and colon were detected by multi-color flow cytometry. induced the strongest innate immune response in gingiva and mononuclear phagocytes (MNPs) changed most significantly, compared to and . Immune dysregulation of the colon was widely induced by . mainly induced immune disorder in spleen. ILC3s, Tregs, CD11B+ dendritic cells s, MNPs, macrophages, and plasmacytoid dendritic cells were the main types in response to key periodontal bacteria. However, the alveolar bone loss was not induced by key periodontal bacteria. In conclusion, the overall immunoregulation of monomicrobial stimuli to decipher the complexities of periodontitis was provided in this study. , , and have different effects on local and systemic immunity in gingiva, colon, and spleen of germ-free mice.
牙周细菌诱导的免疫失调在牙周炎的发展中起重要作用。然而，关键牙周细菌在局部和全身免疫中的作用尚未得到全面研究。在此，为了探索关键牙周细菌的免疫调节图谱，本研究设计了一种用、和单定殖无菌小鼠模型，持续两周。通过显微CT测定牙槽骨吸收情况。用多色流式细胞术检测牙龈、脾脏和结肠中总共14种先天免疫细胞和适应性免疫细胞。与和相比，在牙龈中诱导出最强的先天免疫反应，单核吞噬细胞（MNP）变化最为显著。广泛诱导结肠的免疫失调。主要在脾脏中诱导免疫紊乱。ILC3、调节性T细胞（Treg）、CD11B + 树突状细胞、MNP、巨噬细胞和浆细胞样树突状细胞是对关键牙周细菌产生反应的主要细胞类型。然而，关键牙周细菌未诱导牙槽骨吸收。总之，本研究提供了对单一微生物刺激的整体免疫调节，以解读牙周炎的复杂性。、和对无菌小鼠牙龈、结肠和脾脏的局部和全身免疫有不同影响。
