School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
Int J Biol Macromol. 2020 Dec 1;164:4252-4262. doi: 10.1016/j.ijbiomac.2020.09.018. Epub 2020 Sep 7.
Tissue engineering aims at replacement, repair, and regeneration of tissues by a combination of scaffolds, growth factors, and cells. In this study, we report the synthesis of biodegradable composite scaffolds fortified with mesoporous zinc silicate (mZS) and assessment of in vitro properties for bone tissue engineering (BTE) applications. The scaffolds consisted of chitosan (CS) incorporated with mZS at 0.1, 0.2, 0.3, and 0.5 wt%. The bio-composite scaffolds were visualized using Field Emission Scanning Electron Microscopy (FE-SEM). The incorporation of mZS was confirmed using Energy dispersive x-ray (EDS) analysis. Biomineralization studies were conducted in simulated body fluid (SBF) and indicated bioactivity of fabricated scaffolds. The scaffolds also displayed antibacterial action against Staphylococcus aureus. Cellular attachment within the scaffold network established biocompatibility of the material. Incorporation of mZS within the chitosan scaffolds matrix improved properties such as porosity, degradation rate, and biomineralization. Therefore, fabricated scaffolds exhibit exceptional features and have the potential to serve as an implant for BTE applications.
组织工程旨在通过支架、生长因子和细胞的结合来替代、修复和再生组织。在这项研究中，我们报告了合成了一种具有介孔硅锌（mZS）的可生物降解的复合材料支架，并评估了其在骨组织工程（BTE）应用中的体外性能。支架由壳聚糖（CS）与 0.1、0.2、0.3 和 0.5wt%的 mZS 混合而成。使用场发射扫描电子显微镜（FE-SEM）对生物复合材料支架进行了可视化。通过能量色散 X 射线（EDS）分析证实了 mZS 的掺入。在模拟体液（SBF）中进行了生物矿化研究，表明了所制备支架的生物活性。支架还对金黄色葡萄球菌表现出抗菌作用。支架网络内的细胞附着建立了材料的生物相容性。mZS 掺入壳聚糖支架基质中提高了多孔性、降解速率和生物矿化等性能。因此，所制备的支架具有优异的特性，有可能作为 BTE 应用的植入物。
