Botelho Chirlene N, Falcão Suringo S, Soares Rossy-Eric P, Pereira Silma R, de Menezes Alan S, Kubota Lauro T, Damos Flavio S, Luz Rita C S
Departamento de Química, Laboratório de Sensores, Dispositivos e Métodos Analíticos, Universidade Federal do Maranhão, 65080-805, São Luís, MA, Brazil.
Departamento de Biologia, Laboratório de Genética e Biologia Molecular, Universidade Federal do Maranhão-UFMA, 65080-805, São Luís, MA, Brazil.
Biosens Bioelectron X. 2022 Sep;11:100167. doi: 10.1016/j.biosx.2022.100167. Epub 2022 May 22.
This work aims to develop a photoelectrochemical (PEC) platform for detection of SARS-CoV-2 spike glyprotein S1. The PEC platform is based on the modification of a fluorine-doped tin oxide (FTO) coated glass slide with strontium titanate (SrTiO or ST), sulfur-doped carbon nitride (g-CN-S or CNS) and palladium nanoparticles entrapped in aluminum hydroxide matrix (PdAlO(OH) or PdNPs). The PEC platform was denoted as PdNPs/CNS/ST/FTO and it was characterized by SEM, TEM, FTIR, DRX, and EIS. The PEC response of the PdNPs/CNS/ST/FTO platform was optimized by evaluating the effects of the concentration of the donor molecule, the nature of the buffer, pH, antibody concentration, potential applied to the working electrode, and incubation time. The optimized PdNPs/CNS/ST/FTO PEC platform was modified with 5 μg mL of antibody for determination of SARS-CoV-2 spike glycoprotein S1. A decrease in the photocurrent was observed with an increase in the concentration of SARS-CoV-2 from 1 fg mL to 1000 pg mL showing that the platform is a promising alternative for the detection of S1 protein from SARS-CoV-2. The designed PEC platform exhibited recovery percentages of 96.20% and 109.65% in artificial saliva samples.
这项工作旨在开发一种用于检测新型冠状病毒刺突糖蛋白S1的光电化学（PEC）平台。该PEC平台基于用钛酸锶（SrTiO或ST）、硫掺杂的氮化碳（g-CN-S或CNS）以及包裹在氢氧化铝基质中的钯纳米颗粒（PdAlO(OH)或PdNPs）对氟掺杂氧化锡（FTO）涂层载玻片进行修饰。该PEC平台被标记为PdNPs/CNS/ST/FTO，并通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（DRX）和电化学阻抗谱（EIS）进行表征。通过评估供体分子浓度、缓冲液性质、pH值、抗体浓度、施加于工作电极的电位以及孵育时间的影响，对PdNPs/CNS/ST/FTO平台的PEC响应进行了优化。用5μg/mL的抗体对优化后的PdNPs/CNS/ST/FTO PEC平台进行修饰，以测定新型冠状病毒刺突糖蛋白S1。随着新型冠状病毒浓度从1fg/mL增加到1000pg/mL，观察到光电流降低，这表明该平台是检测新型冠状病毒S1蛋白的一种有前景的替代方法。所设计的PEC平台在人工唾液样本中的回收率分别为96.20%和109.65%。
