色素颗粒在色素性青光眼 DBA/2J 小鼠模型水样房水引流结构中的分布。

Distribution of Pigment Particles in Aqueous Drainage Structures in a DBA/2J Mouse Model of Pigmentary Glaucoma.

机构信息

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing, China.

出版信息

Invest Ophthalmol Vis Sci. 2022 Jun 1;63(6):2. doi: 10.1167/iovs.63.6.2.

DOI:10.1167/iovs.63.6.2

PMID:35648639

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9172048/

Abstract

PURPOSE

To characterize the distribution of pigment particles in aqueous drainage structures of DBA/2J mice with different intraocular pressure (IOP) levels.

METHODS

DBA/2J mice were monitored from 9 to 44 weeks of age. IOP measurements were performed periodically. At 12, 20, 28, and 36 weeks, three mice were randomly selected for each time point and divided into three IOP groups. The morphology, size, and quantity of pigment particles in aqueous drainage structures were determined via transmission electron microscopy combined with ImageJ-based analysis. Between-group differences were evaluated with a one-way analysis of variance and Fisher's least significant difference test.

RESULTS

In the anterior chamber, 74.2% (187/252) of pigment particles were round (diameter range, 0.20-0.73 µm), and 25.8% (65/252) were oval (length range, 0.35-1.20 µm ). In the high-IOP group (IOP≥15 mmHg), pigment particles in the trabecular meshwork (TM) were more abundant and larger in size than those in the normal-IOP group (P<0.001). All separate pigment particles in the TM of the high-IOP group were >0.4 µm in size. The diameters of round (IOP≤10 mmHg, 0.44±0.13 µm; IOP between 10 and 15 mmHg, 0.57±0.13 µm; IOP≥15 mmHg, 0.61±0.12 µm) and the lengths of oval (0.65±0.14 µm vs. 0.77±0.12 µm vs. 0.88±0.15 µm, respectively) pigment particles in the TM differed among groups (F=27.258 and F=27.295, respectively; both P<0.001). No such differences were discovered in the iris and around Schlemm's canal (P>0.05).

CONCLUSIONS

In DBA/2J mice, large and medium pigment particles (>0.4 µm) seem to play an important role in causing aqueous outflow obstruction and IOP elevation.

摘要

目的

描述不同眼压（IOP）水平的 DBA/2J 小鼠房水引流结构中色素颗粒的分布情况。

方法

从 9 周到 44 周龄监测 DBA/2J 小鼠。定期进行 IOP 测量。在 12、20、28 和 36 周时，每个时间点随机选择 3 只小鼠，并将其分为 3 个 IOP 组。通过透射电子显微镜结合基于 ImageJ 的分析方法，确定房水引流结构中色素颗粒的形态、大小和数量。采用单因素方差分析和 Fisher 最小显著差异检验评估组间差异。

结果

在前房，74.2%（187/252）的色素颗粒为圆形（直径范围为 0.20-0.73 µm），25.8%（65/252）为椭圆形（长度范围为 0.35-1.20 µm）。在高眼压组（IOP≥15 mmHg）中，小梁网中的色素颗粒比正常眼压组更丰富且更大（P<0.001）。高眼压组小梁网中的所有单独色素颗粒均>0.4 µm。圆形色素颗粒的直径（IOP≤10 mmHg，0.44±0.13 µm；IOP 在 10 和 15 mmHg 之间，0.57±0.13 µm；IOP≥15 mmHg，0.61±0.12 µm）和椭圆形色素颗粒的长度（0.65±0.14 µm 比 0.77±0.12 µm 比 0.88±0.15 µm）在各组之间存在差异（F=27.258 和 F=27.295，均 P<0.001）。在虹膜和 Schlemm 管周围未发现这种差异（P>0.05）。