Li Jinxing, Liu Yuxin, Yuan Lei, Zhang Baibing, Bishop Estelle Spear, Wang Kecheng, Tang Jing, Zheng Yu-Qing, Xu Wenhui, Niu Simiao, Beker Levent, Li Thomas L, Chen Gan, Diyaolu Modupeola, Thomas Anne-Laure, Mottini Vittorio, Tok Jeffrey B-H, Dunn James C Y, Cui Bianxiao, Pașca Sergiu P, Cui Yi, Habtezion Aida, Chen Xiaoke, Bao Zhenan
Department of Chemical Engineering, Stanford University, Stanford, CA, USA.
Department of Biomedical Engineering and Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, USA.
Nature. 2022 Jun;606(7912):94-101. doi: 10.1038/s41586-022-04615-2. Epub 2022 Jun 1.
Neurotransmitters play essential roles in regulating neural circuit dynamics both in the central nervous system as well as at the peripheral, including the gastrointestinal tract. Their real-time monitoring will offer critical information for understanding neural function and diagnosing disease. However, bioelectronic tools to monitor the dynamics of neurotransmitters in vivo, especially in the enteric nervous systems, are underdeveloped. This is mainly owing to the limited availability of biosensing tools that are capable of examining soft, complex and actively moving organs. Here we introduce a tissue-mimicking, stretchable, neurochemical biological interface termed NeuroString, which is prepared by laser patterning of a metal-complexed polyimide into an interconnected graphene/nanoparticle network embedded in an elastomer. NeuroString sensors allow chronic in vivo real-time, multichannel and multiplexed monoamine sensing in the brain of behaving mouse, as well as measuring serotonin dynamics in the gut without undesired stimulations and perturbing peristaltic movements. The described elastic and conformable biosensing interface has broad potential for studying the impact of neurotransmitters on gut microbes, brain-gut communication and may ultimately be extended to biomolecular sensing in other soft organs across the body.
神经递质在调节中枢神经系统以及包括胃肠道在内的外周神经回路动力学方面发挥着重要作用。对它们的实时监测将为理解神经功能和诊断疾病提供关键信息。然而，用于监测体内神经递质动态的生物电子工具，尤其是在肠神经系统中，仍未得到充分发展。这主要是由于能够检测柔软、复杂且活跃移动器官的生物传感工具有限。在此，我们介绍一种名为NeuroString的组织模拟、可拉伸的神经化学生物界面，它是通过将金属络合聚酰亚胺激光图案化为嵌入弹性体中的互连石墨烯/纳米颗粒网络而制备的。NeuroString传感器能够在行为小鼠的大脑中进行慢性体内实时、多通道和多重单胺传感，还能在不产生不必要刺激和干扰蠕动的情况下测量肠道中的血清素动态。所描述的弹性且贴合的生物传感界面在研究神经递质对肠道微生物的影响、脑-肠通信方面具有广泛潜力，并且最终可能扩展到全身其他柔软器官的生物分子传感。
