Department of Stomatology, Nanfang Hospital, Southern Medical University, Shatainan Road, Baiyun District, Guangzhou, 510515, Guangdong, China.
School of Stomatology, Southern Medical University, Guangzhou, Guangdong, China.
Odontology. 2022 Oct;110(4):697-709. doi: 10.1007/s10266-022-00715-7. Epub 2022 Jun 3.
The destruction of alveolar bone is a crucial manifestation of severe chronic periodontitis, which stem cell-based bioengineered therapies are expected to cure. Therefore, a cost-effective, reproducible, quantifiability and easier to administrate animal model that mimics human periodontitis is of great importance for further endeavor. In this study, we created periodontitis rat models in silk ligation group, bone defect group and bone defect/silk ligation group, respectively. Obvious periodontal inflammation but slight alveolar bone resorption was observed in the ligation group, while surgical trauma was not robust enough to continually worsen the constructed bone defect area in the bone defect group. In the bone defect/ligature group, significant and stable periodontal inflammation was the most enduring with similar evolving pathological patterns of human periodontitis. It also exhibited enhanced clinical similarity and confirmed its superiority in quantitativeness. The present rat model is the first study to reproduce a pathological process similar to human periodontitis with reliable stability and repeatability, manifesting a priority to previous methods. Day 9-12 is the best time for reproducing severe periodontitis syndromes with vertical bone resorption in this model.
牙槽骨破坏是重度慢性牙周炎的一个关键表现，基于干细胞的生物工程疗法有望对此进行治疗。因此，建立一种具有成本效益、可重复性、可量化性和更易于管理的、模拟人类牙周炎的动物模型对于进一步的研究至关重要。在这项研究中，我们分别在丝线结扎组、骨缺损组和骨缺损/丝线结扎组中建立了牙周炎大鼠模型。结扎组观察到明显的牙周炎症，但牙槽骨吸收轻微，而骨缺损组的手术创伤不足以持续加重构建的骨缺损区域。在骨缺损/结扎组中，最持久的是显著且稳定的牙周炎症，其发展的病理模式与人类牙周炎相似。该模型还表现出增强的临床相似性，并证实了其在定量方面的优势。本大鼠模型是第一个能够重现类似于人类牙周炎的病理过程的研究，具有可靠的稳定性和可重复性，优于之前的方法。在该模型中，第 9-12 天是复制具有垂直骨吸收的重度牙周炎综合征的最佳时间。
