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基于嗜酸乳杆菌干预的 lncRNA-miRNA-mRNA 网络探讨直肠癌新靶点：一项随机临床试验。

Novel targets in rectal cancer by considering lncRNA-miRNA-mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial.

机构信息

Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.

Evidence-Based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran.

出版信息

Sci Rep. 2022 Jun 2;12(1):9168. doi: 10.1038/s41598-022-13297-9.

DOI:10.1038/s41598-022-13297-9
PMID:35654932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9163174/
Abstract

We aimed to explore the lncRNA-miR-mRNA network in response to Lactobacillus acidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA-miR-mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L. acidophilus consumption was associated with improved expression of the lncRNA-miR-mRNA network, which may provide novel monitoring and therapeutic approaches.

摘要

我们旨在探讨嗜酸乳杆菌（L. acidophilus）消耗对直肠癌患者中 lncRNA-miR-mRNA 网络的影响。首先从 GEO 和 TCGA 数据库中提取候选 miR。我们使用高通量测序数据构建了 lncRNA-miR-mRNA 网络。最后，我们根据实验数据创建了一个热图，以显示所选靶标的可能相关性。使用 Real-Time PCR 测量了 107 名接受安慰剂和益生菌消耗以及 10 名非癌症患者的直肠癌细胞中选定靶标。我们的分析显示了一组在直肠癌患者中差异表达的 12 个 miRs 和 11 个 lncRNAs 和 12 个基因。与安慰剂组相比，益生菌消耗后，所选肿瘤抑制 miR、lncRNA 和基因的表达显著增加，而所选致癌 miR、oncoclncRNA 和致癌基因的表达显著降低。网络成分之间存在很强的相关性，包括 miR-133b 和 IGF1 基因、miR-548ac 和 MSH2 基因以及 miR-21 和 SMAD4 基因。在直肠癌患者中，嗜酸乳杆菌的消耗与 lncRNA-miR-mRNA 网络表达的改善有关，这可能为监测和治疗提供新的方法。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a03/9163174/42e910a6546f/41598_2022_13297_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a03/9163174/cde783c6e284/41598_2022_13297_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a03/9163174/6e1dd594ce6c/41598_2022_13297_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a03/9163174/e7e106693e91/41598_2022_13297_Fig9_HTML.jpg
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