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利用非变性荧光原位杂交技术对国际玉米小麦改良中心和中国小麦核心种质进行遗传多样性评估

Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization.

作者信息

Yang Manyu, Yang Zujun, Yang Wuyun, Yang Ennian

机构信息

Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.

Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs of P.R.C.), Chengdu 610066, China.

出版信息

Plants (Basel). 2022 May 25;11(11):1403. doi: 10.3390/plants11111403.

DOI:10.3390/plants11111403
PMID:35684176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9183173/
Abstract

Germplasm is the material basis for crop genetic improvement and related basic research. Knowledge of genetic diversity present in wheat is the prerequisite for wheat breeding and improvement. Non-denaturing fluorescence in situ hybridization (ND-FISH) is a powerful tool to distinguish chromosomal polymorphisms and evaluate genetic diversity in wheat. In this study, ND-FISH using Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)7 as probes were used to analyze the genetic diversity among 60 International Maize and Wheat Improvement Center (CIMMYT) derived wheat lines, and 93 cultivated wheat and landraces from the Chinese wheat core germplasm. A total of 137 polymorphic FISH patterns were obtained, in which 41, 65, and 31 were from A-, B-, and D-genome chromosomes, respectively, indicating polymorphism of B-genome > A-genome > D-genome. In addition, 22 and 51 specific FISH types were observed in the two germplasm resource lines. Twelve types of rearrangements, including seven new translocations, were detected in all 153 wheat lines. Genetic relationships among 153 wheat lines were clustered into six groups. Our research provides cytological information for rational utilization of wheat germplasm resources.

摘要

种质是作物遗传改良及相关基础研究的物质基础。了解小麦中存在的遗传多样性是小麦育种与改良的前提。非变性荧光原位杂交（ND-FISH）是区分染色体多态性和评估小麦遗传多样性的有力工具。在本研究中，以Oligo-pSc119.2-1、Oligo-pTa535-1和Oligo-(GAA)7为探针的ND-FISH被用于分析60份来自国际玉米小麦改良中心（CIMMYT）的小麦品系以及93份来自中国小麦核心种质的栽培小麦和地方品种之间的遗传多样性。共获得137种多态性FISH模式，其中分别有41、65和31种来自A、B、D基因组染色体，表明B基因组的多态性>A基因组>D基因组。此外，在两个种质资源品系中观察到22种和51种特异性FISH类型。在所有153个小麦品系中检测到12种重排类型，包括7种新的易位。153个小麦品系之间的遗传关系被聚类为6组。我们的研究为合理利用小麦种质资源提供了细胞学信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/db65be5cd6cf/plants-11-01403-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/1c8870fdecdc/plants-11-01403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/dd89157305de/plants-11-01403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/2560950989b8/plants-11-01403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/2144bc6ed8bc/plants-11-01403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/4ac8464327e3/plants-11-01403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/9e6f3732ee74/plants-11-01403-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/c9e123e0d1b9/plants-11-01403-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/1866f2fc9e1d/plants-11-01403-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/db65be5cd6cf/plants-11-01403-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/1c8870fdecdc/plants-11-01403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/dd89157305de/plants-11-01403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/2560950989b8/plants-11-01403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/2144bc6ed8bc/plants-11-01403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/4ac8464327e3/plants-11-01403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/9e6f3732ee74/plants-11-01403-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/c9e123e0d1b9/plants-11-01403-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/1866f2fc9e1d/plants-11-01403-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f95/9183173/db65be5cd6cf/plants-11-01403-g009.jpg

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本文引用的文献

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Multiple wheat genomes reveal global variation in modern breeding.多个小麦基因组揭示了现代育种中的全球变异。
Nature. 2020 Dec;588(7837):277-283. doi: 10.1038/s41586-020-2961-x. Epub 2020 Nov 25.
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Molecular genetic analysis of spring wheat core collection using genetic diversity, population structure, and linkage disequilibrium.
利用遗传多样性、群体结构和连锁不平衡对春小麦核心种质进行分子遗传分析。
BMC Genomics. 2020 Jun 26;21(1):434. doi: 10.1186/s12864-020-06835-0.
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Genetic diversity and population structure analysis of synthetic and bread wheat accessions in Western Siberia.西伯利亚西部的合成小麦和面包小麦品种的遗传多样性和群体结构分析。
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