Su Danyan, Huang Yanyun, Liu Dongli, Huang Yuqin, Ye Bingbing, Qin Suyuan, Chen Cheng, Pang Yusheng
Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Transl Pediatr. 2022 May;11(5):715-727. doi: 10.21037/tp-22-117.
Circular RNAs (circRNAs) may play important roles in the progression of pulmonary arterial hypertension. However, the potential roles they play in childhood pulmonary arterial hypertension associated congenital heart disease (CHD) progression remains unclear.
Thirteen human plasma samples including eight from pulmonary arterial hypertension secondary to CHD patients and five from a control group were analyzed using the Arraystar Human circRNA array. The relative expression levels of five differentially expressed circRNAs in pulmonary arterial hypertension were detected using real-time polymerase chain reaction (PCR) analysis. In parallel, these levels were also taken on control samples from 32 CHD patients. We used miRanda and TargetScan software packages to predict potential microRNA (miRNA)targets, which were then combined into a circRNA-miRNA-messenger RNA (mRNA) network.
Twenty-seven circRNAs (three upregulated and 24 downregulated) were differentially expressed between the pulmonary arterial hypertension and control groups. Compared to control group levels, circ_003416 expression in the pulmonary arterial hypertension group was significantly downregulated, while circ_005372 expression, in contrast, was significantly upregulated. The differential expression of these circRNAs was mainly linked to variation in levels of oxidative phosphorylation and tight junction signaling.
We identified one overexpressed and one underexpressed circRNA in plasma samples from children with CHD associated pulmonary arterial hypertension. Bioinformatic analysis indicated these dysregulated circRNAs might be associated with the occurrence and regulation of pulmonary arterial hypertension.
环状RNA（circRNA）可能在肺动脉高压的进展中发挥重要作用。然而，它们在儿童先天性心脏病（CHD）相关肺动脉高压进展中所起的潜在作用仍不清楚。
使用Arraystar人类circRNA芯片分析了13份人类血浆样本，其中8份来自CHD继发肺动脉高压患者，5份来自对照组。采用实时聚合酶链反应（PCR）分析检测肺动脉高压中5种差异表达circRNA的相对表达水平。同时，也对32例CHD患者的对照样本进行了检测。我们使用miRanda和TargetScan软件包预测潜在的微小RNA（miRNA）靶点，然后将其整合到circRNA-miRNA-信使RNA（mRNA）网络中。
肺动脉高压组和对照组之间有27种circRNA（3种上调和24种下调）差异表达。与对照组水平相比，肺动脉高压组中circ_003416的表达显著下调，而circ_005372的表达则显著上调。这些circRNA的差异表达主要与氧化磷酸化和紧密连接信号水平的变化有关。
我们在CHD相关肺动脉高压患儿的血浆样本中鉴定出一种过表达和一种低表达的circRNA。生物信息学分析表明，这些失调的circRNA可能与肺动脉高压的发生和调控有关。
