NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Yantai Center for Disease Control and Prevention, Yantai, China.
Front Public Health. 2022 May 25;10:819890. doi: 10.3389/fpubh.2022.819890. eCollection 2022.
This study aimed to assess the correlation between Norovirus (NoV), diarrhea, and raw oysters from the eastern coastal areas of Yantai, Shandong, China.
Marine oysters were selected from the three aquatic markets in Laishan district, Yantai City, in March 2019. Meanwhile, 100 fecal samples were collected from patients with diarrhea from the same areas during the same period. Nucleic acids were extracted from these samples and detected by employing reverse transcription polymerase chain reaction (RT-PCR) for NoV GI/GII. The VP1 gene of the coat protein of NoV was amplified by semi-nested RT-PCR and sequenced. Sequence comparison of VP1 was performed with BioEdit software, and the evolutionary tree was constructed with Mega7.0 software.
Of the 151 oysters, 42 (27.8%) were positive for NoV. Among them, 32 (21.2%) were GII-positive, 10 (6.6%) were GI-positive, and one GI VP1 sequence was obtained in the oyster samples. Of 100 fecal samples from patients with diarrhea, 38 were GII-positive and 17 were GI-positive. Totally, 19 GII VP 1 sequences and eight GI VP 1 sequences were obtained. Two G1 VP 1 sequences in two fecal samples showed 98.7% nucleotide sequence identity and 99.1% amino acid sequence identity G1 VP 1 acquired in the oyster sample.
The results suggest that oysters may be responsible for the spread of NoV in Yantai, Shandong province, China.
本研究旨在评估中国山东烟台东部沿海地区诺如病毒（NoV）、腹泻和生牡蛎之间的相关性。
2019 年 3 月，从烟台市莱山区三个水产市场中选择了牡蛎。同时，同期从同一地区的 100 例腹泻患者中采集了 100 份粪便样本。从这些样本中提取核酸，采用逆转录聚合酶链反应（RT-PCR）检测 NoV GI/GII。采用半巢式 RT-PCR 扩增 NoV 外壳蛋白的 VP1 基因并测序。利用 BioEdit 软件对 VP1 进行序列比较，利用 Mega7.0 软件构建进化树。
在 151 个牡蛎中，有 42 个（27.8%）检测到 NoV 呈阳性。其中，32 个（21.2%）为 GII 阳性，10 个（6.6%）为 GI 阳性，在牡蛎样本中获得了一个 GI VP1 序列。在 100 份腹泻患者粪便样本中，有 38 份为 GII 阳性，17 份为 GI 阳性。共获得 19 个 GII VP1 序列和 8 个 GI VP1 序列。在两个粪便样本中获得的两个 G1 VP1 序列与在牡蛎样本中获得的 G1 VP1 具有 98.7%的核苷酸序列同一性和 99.1%的氨基酸序列同一性。
结果表明，牡蛎可能是导致中国山东烟台地区诺如病毒传播的原因。
