Banala Sambashiva, Tkachuk Ariana N, Patel Ronak, Kumar Pratik, Brown Timothy A, Lavis Luke D
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, United States.
ACS Bio Med Chem Au. 2022 Jun 15;2(3):307-312. doi: 10.1021/acsbiomedchemau.1c00068. Epub 2022 Feb 28.
Small-molecule fluorescent stains enable the imaging of cellular structures without the need for genetic manipulation. Here, we introduce 2,7-diaminobenzopyrylium (DAB) dyes as live-cell mitochondrial stains excited with violet light. This amalgam of the coumarin and rhodamine fluorophore structures yields dyes with high photostability and tunable spectral properties.
小分子荧光染料能够对细胞结构进行成像，而无需进行基因操作。在此，我们引入2,7-二氨基苯并吡喃鎓（DAB）染料作为用紫光激发的活细胞线粒体染料。这种香豆素和罗丹明荧光团结构的融合产生了具有高光稳定性和可调光谱特性的染料。
