Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne, Switzerland.
Nat Chem. 2013 Feb;5(2):132-9. doi: 10.1038/nchem.1546. Epub 2013 Jan 6.
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.
用于生物成像的理想荧光探针应该具有明亮、长波长吸收和能够在活细胞和体内灵活实现的特点。然而，设计结合所有这些特性的合成荧光团被证明是极其困难的。在这里，我们介绍了一种生物相容性的近红外硅罗丹明探针，可以使用不同的标记技术特异性地与蛋白质偶联。重要的是，它的高通透性和荧光特性使其能够对活细胞和组织中的蛋白质进行成像，其亮度和光稳定性使其非常适合活细胞超分辨率显微镜。该探针具有优异的光谱特性，并且易于在活细胞应用中使用，因此成为生物成像的有力新工具。
